Lectin-binding substance measurement method, lectin-binding substance measurement kit, and capture carrier for use in these

Patent metadata

Field	Value
Publication number	US-12038440-B2
Application number	US-202017639400-A
Country	US
Kind code	B2
Filing date	Sep 1, 2020
Priority date	Sep 2, 2019
Publication date	Jul 16, 2024
Grant date	Jul 16, 2024

Abstract

A lectin-binding substance measurement method is a method for measuring a lectin-binding substance in a sample, and includes: a capturing step of bringing a capture carrier including a water-insoluble carrier and a molecule immobilized on the water-insoluble carrier, the molecule being a molecule capable of capturing a lectin-binding substance, into contact with the sample to cause the capture carrier to capture the lectin-binding substance; a washing step of removing contaminants unbound to the capture carrier; a releasing step of releasing the lectin-binding substance from the capture carrier to obtain a prepared sample; and a measuring step of measuring the lectin-binding substance in the prepared sample by using a lectin.

First claim

The invention claimed is:

1. A lectin-binding substance measurement method for measuring a lectin-binding substance in a sample, the method comprising:
purifying the lectin-binding substance by steps including at least
a capturing step of bringing a capture carrier including a water-insoluble carrier and a molecule immobilized on the water-insoluble carrier, the molecule being a molecule capable of capturing a lectin-binding substance, into contact with the sample to cause the capture carrier to capture the lectin-binding substance;
a washing step of removing contaminants unbound to the capture carrier; and
a releasing step of releasing the lectin-binding substance from the capture carrier to obtain a prepared sample; and

measuring the purified lectin-binding substance in the prepared sample by using a lectin, wherein
the lectin is at least one of
a labeled lectin including a labeling substance and a lectin, and
a blocked labeled lectin including a water-soluble carrier made of a first water-soluble polymer, a labeling substance and a lectin immobilized on the water-soluble carrier,

the lectin-binding substance is a substance having a glycan capable of binding to a lectin,
the molecule capable of capturing the lectin-binding substance is a molecule capable of recognizing and binding to a portion other than the glycan portion in the lectin-binding substance, and
the capture carrier does not include the labeling substance.

2. The lectin-binding substance measurement method according to claim 1 , wherein
the measuring includes a step of bringing the labeled lectin into contact with the prepared sample.

3. The lectin-binding substance measurement method according to claim 1 , wherein
the measuring includes a step of bringing the blocked labeled lectin into contact with the prepared sample.

4. The lectin-binding substance measurement method according to claim 1 , wherein the measuring is carried out in the presence of a free second-water-soluble polymer.

Assignees

• Fujirebio Kk

Inventors

• Okada Kazunori
• Iida Kumiko
• Yagi Shintaro
• Aoyagi Katsumi

Classifications

• G01N33/5758 Primary

  involving compounds serving as markers for tumours, cancers or neoplasias, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides or metabolites · CPC title

• G01N2333/471

  Pregnancy proteins, e.g. placenta proteins, alpha-feto-protein, pregnancy specific beta glycoprotein · CPC title

• G01N2333/4724

  Lectins · CPC title

• G01N33/543 Primary

  with an insoluble carrier for immobilising immunochemicals · CPC title

• G01N33/57484 Primary

  Physics · mapped topic