Methods for preparing N-acetyl-L-methionine

Patent metadata

Field	Value
Publication number	US-12037610-B2
Application number	US-202117452565-A
Country	US
Kind code	B2
Filing date	Oct 27, 2021
Priority date	Jul 20, 2016
Publication date	Jul 16, 2024
Grant date	Jul 16, 2024

Abstract

The present disclosure relates to a polypeptide having an acyltransferase activity or a microorganism including the same; a composition for preparing N-acetyl-L-methionine, the composition including the polypeptide or microorganism; and a method of preparing N-acetyl-L-methionine using the polypeptide or microorganism. Further, the present disclosure relates to a polynucleotide encoding the polypeptide and an expression vector including the polynucleotide. Since the microorganism including a novel acyltransferase according to the present disclosure has enhanced acyltransferase activity, this microorganism can be efficiently used for producing N-acetyl-L-methionine by acetylating L-methionine.

First claim

The invention claimed is:

1. A method of preparing N-acetyl-L-methionine, comprising:
acetylating L-methionine using (A) a microorganism producing N-acetyl-L-methionine from L-methionine, the microorganism comprising a polypeptide having an acyltransferase activity and having (i) an amino acid sequence identical to any one of SEQ ID NOS. 1-3 or (ii) an amino acid sequence having 90% or more homology to one of such amino acid sequences or (B) a culture of the microorganism,
wherein the microorganism is selected from the group consisting of an Escherichia sp., Corynebacterium sp., Saccharomyces sp., and Yarrowias p.

2. The method according to claim 1 , further comprising:
recovering N-acetyl-L-methionine, which is the acetylated L-methionine.

3. A method of preparing N-acetyl-L-methionine, comprising: acetylating L-methionine using (A) a microorganism producing N-acetyl-L-methionine from L-methionine, the microorganism comprising a polypeptide having an acyltransferase activity and having (i) an amino acid sequence identical to any one of SEQ ID NOS. 1-3 or (ii) an amino acid sequence having 90% or more homology to one of such amino acid sequences or (B) a culture of the microorganism, wherein the microorganism is an Escherichia sp.

4. The method of claim 3 , comprising: acetylating L-methionine using (A) a microorganism producing N-acetyl-L-methionine from L-methionine, the microorganism comprising a polypeptide having an acyltransferase activity and having an amino acid sequence identical to any one of SEQ ID NOS. 1-3 or (B) a culture of the microorganism, wherein the microorganism is an Escherichia sp.

5. The method according to claim 4 , further comprising recovering N-acetyl-L-methionine, which is the acetylated L-methionine.

Assignees

• Cj Cheiljedang Corp

Inventors

• Hong So Yeon
• Park Jinseung
• Park Hye Min
• Choi Su Jin
• Moon Jun Ok
• Lee Sung Gun
• Jeon Jinwoo

Classifications

• C12N9/10

  Transferases (2.) (ribonucleases C12N9/22) · CPC title

• C12N15/63 Primary

  Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression · CPC title

• C12Y203/01001

  Amino-acid N-acetyltransferase (2.3.1.1) · CPC title

• C12P13/12 Primary

  Methionine; Cysteine; Cystine · CPC title

• C12N15/81

  for yeasts · CPC title