Modification of transcriptional repressor binding site in NF-YC4 promoter for increased protein content and resistance to stress

Patent metadata

Field	Value
Publication number	US-12037594-B2
Application number	US-202218057439-A
Country	US
Kind code	B2
Filing date	Nov 21, 2022
Priority date	Feb 18, 2015
Publication date	Jul 16, 2024
Grant date	Jul 16, 2024

Abstract

Method of increasing protein content in a eukaryotic cell comprising an NF-YC4 gene comprising modifying the transcriptional repressor binding site; method of producing a plant with increased protein content comprising crossing and selecting for increased protein content; method of increasing resistance to a pathogen or a pest in a plant cell or plant comprising an NF-YC4 gene comprising modifying the transcriptional repressor binding site, alone or in further combination with expressing QQS in the plant cell or plant; method for producing a plant with increased resistance to a pathogen or a pest comprising crossing and selecting for increased resistance to the pathogen or the pest; a cell, collection of cells, tissue, organ, or organism, such as a plant, in which the NF-YC4 gene comprises a promoter comprising a transcriptional repressor binding site that has been modified so that the transcriptional repressor cannot prevent transcription of the NF-YC4; plants and hybrids thereof; and seeds.

First claim

What is claimed is:

1. A genetically modified plant, or a part thereof, transformed with a nucleic acid molecule comprising a nucleic acid sequence encoding a nuclear factor Y subunit C4 (NF-YC4) protein, wherein said nucleic acid sequence is operably linked to a heterologous promoter to overexpress said NF-YC4 protein in said genetically modified plant, or said part thereof, and wherein said genetically modified plant or said genetically modified part thereof transformed with said nucleic acid molecule was selected for increased total protein content due to overexpression of said NE-YC4 protein as compared to the total protein content in the corresponding wild-type (WT) plant or the corresponding WT part thereof of the same plant species, which does not comprise said nucleic acid molecule.

2. The genetically modified plant, or the part thereof, of claim 1 , wherein the total protein content is increased in the leaves or the seeds of the plant or the part thereof.

3. The genetically modified plant, or the part thereof, of claim 1 , wherein the plant or the part thereof is a crop plant.

4. The genetically modified plant, or the part thereof, of claim 1 , wherein the crop plant is soybean, rice, or corn.

5. The genetically modified plant, or the part thereof, of claim 1 , wherein the plant or the part thereof is a monocot.

6. The genetically modified plant, or the part thereof, of claim 1 , wherein the plant or the part thereof is a dicot.

7. The genetically modified plant, or the part thereof, of claim 1 , wherein the crop plant is selected from the group consisting of corn, soybean, alfalfa, canola, wheat, rice, potato and lettuce.

8. The genetically modified plant, or the part thereof, of claim 1 , wherein the NF-YC4 protein is selected from the group consisting of Brassica napus NF-YC4 protein Medicago truncatula NF-YC4 protein Arabidopsis thaliana NF-YC4 protein Glycine max NF-YC4 protein Oryza sativa NF-YC4 protein, and Zea mays NF-YC4 protein.

9. The genetically modified plant, or the part thereof, of claim 1 , wherein the promoter is a cauliflower mosaic virus promoter.

10. The genetically modified plant, or the part thereof, of claim 9 , wherein the cauliflower mosaic virus promoter is the p35s promoter.

11. A genetically modified plant progeny obtained from the genetically modified plant, or the part thereof, of claim 1 , wherein said genetically modified plant progeny comprises said nucleic acid molecule and exhibits increasing protein content as compared to a corresponding wild-type control plant of the species that lacks said nucleic acid molecule.

Assignees

• Univ Iowa State Res Found Inc

Inventors

• Li Ling
• Wurtele Eve Syrkin

Classifications

• C12N15/8279

  for biotic stress resistance, pathogen resistance, disease resistance · CPC title

• C12N15/8216

  Methods for controlling, regulating or enhancing expression of transgenes in plant cells · CPC title

• C12N15/8205

  Agrobacterium mediated transformation · CPC title

• C12N15/8241

  Phenotypically and genetically modified plants via recombinant DNA technology · CPC title

• C12N15/8247

  involving modified lipid metabolism, e.g. seed oil composition · CPC title