System and method for creating tissue

Patent metadata

Field	Value
Publication number	US-12024701-B2
Application number	US-202117465133-A
Country	US
Kind code	B2
Filing date	Sep 2, 2021
Priority date	Nov 7, 2016
Publication date	Jul 2, 2024
Grant date	Jul 2, 2024

Abstract

A system and method for growing and maintaining biological material including producing a protein associated with the tissue, selecting cells associated with the tissue, expanding the cells, creating at least one tissue bio-ink including the expanded cells, printing the at least one tissue bio-ink in at least one tissue growth medium mixture, growing the tissue from the printed at least one tissue bio-ink, and maintaining viability of the tissue.

First claim

The invention claimed is:

1. A method for automatically growing tissue using a system having a controller, the tissue having tissue characteristics, the method comprising the controller:
selecting, from a tissue sample, cells associated with the tissue, based on the tissue characteristics;
expanding the selected cells;
creating at least one printable protein bio-ink;
creating at least one protein from the at least one printable protein bio-ink;
creating at least one tissue bio-ink including the expanded cells, the bio-ink, and the at least one protein;
creating at least one tissue growth medium mixture including the at least one protein;
printing the at least one tissue bio-ink in the at least one tissue growth medium mixture;
growing the tissue from the printed at least one tissue bio-ink in the at least one tissue growth medium mixture; and
maintaining viability of the tissue.

2. The method as in claim 1 wherein the tissue growth medium mixture further comprises:
indicators, support materials, gel, and basal medium.

3. The method as in claim 1 wherein the tissue growth medium mixture further comprises:
indicators, support materials, carbomer, and basal media.

4. The method as in claim 1 maintaining the viability comprises:
evacuating wastes from metabolism of the tissue.

Assignees

• Deka Products Lp

Inventors

• Langenfeld Christopher C
• Cannan David D B
• Van Der Merwe Dirk A
• Kamen Dean
• Demers Jason A
• Morgan Frederick
• Moreau Timothy D
• Tracey Brian D
• Ware Matthew
• Lanigan Richard J
• Baker Michael A
• Blumberg Jr David
• Gautney Richard E
• Kane Derek G
• Fawkes Dane
• Bollenbach Thomas J
• Tilley Michael C
• Jacobson Stuart A
• Mannisto John F

Classifications

• A01N1/143

  for organ perfusion · CPC title

• C12N2533/00

  Supports or coatings for cell culture, characterised by material · CPC title

• C12N2533/90

  Substrates of biological origin, e.g. extracellular matrix, decellularised tissue · CPC title

• C12N2533/50

  Proteins · CPC title

• C12N5/0062

  General methods for three-dimensional culture · CPC title