Nanopore biosensors for detection of proteins and nucleic acids
US-2016053300-A1 · Feb 25, 2016 · US
Transmembrane pore consisting of two CsgG pores
US12024541B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12024541-B2 |
| Application number | US-201816610895-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 3, 2018 |
| Priority date | May 4, 2017 |
| Publication date | Jul 2, 2024 |
| Grant date | Jul 2, 2024 |
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- Title
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Abstract
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Provided is a method of characterising a polynucleotide using a transmembrane pore, wherein the pore is a double pore comprising a first Csg G pore, or a homologue thereof, and a second CsgG pore, or a homologue thereof.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method of characterising a polynucleotide using a transmembrane pore, the method comprising translocating the polynucleotide through the transmembrane pore, wherein the transmembrane pore comprises a first CsgG pore, or a homologue thereof, and a second CsgG pore, or a homologue thereof, arranged vertically to form a channel through a membrane where the tail of the first CsgG pore associates with the tail of the second CsgG pore. 2. A method according to claim 1 , wherein the polynucleotide comprises a homopolymeric region. 3. A method according to claim 1 , wherein the first CsgG pore, or homologue thereof, is a homooligomer and the second CsgG pore, or homologue thereof, is a homooligomer. 4. The method of claim 3 , wherein the first CsgG pore, or homologue thereof, comprises monomers that have a different amino acid sequence from the monomers of which the second CsgG pore, or homologue thereof, is comprised. 5. The method of claim 1 , wherein the first CsgG pore, or homologue thereof, is a homooligomer and the second CsgG pore, or homologue thereof, is a homooligomer, and the first CsgG pore, or homologue thereof, and/or the second CsgG pore, or homologue thereof is not a wild-type CsgG pore. 6. The method of claim 5 , wherein the first CsgG pore or homologue thereof, and the second CsgG pore, or homologue thereof comprise identical amino acid sequences. 7. The method of claim 1 , wherein the first CsgG pore, or homologue thereof, is a heterooligomer and the second CsgG pore, or homologue thereof, is a homooligomer. 8. The method of claim 1 , wherein the first CsgG pore, or homologue thereof, is a homooligomer and the second CsgG pore, or homologue thereof, is a heterooligomer. 9. The method of claim 1 , wherein the first CsgG pore, or homologue thereof, is a heterooligomer and the second CsgG pore, or homologue thereof, is a heterooligomer. 10. The method of claim 9 , wherein the first CsgG pore, or homologue thereof, and the second CsgG pore, or homologue thereof comprise identical amino acid sequences.
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Classifications
Investigating individual macromolecules, e.g. by translocation through nanopores (Coulter counters in general G01N15/12; fabrication methods for nanoscale apertures B81B1/00; sequencing of nucleic acids C12Q1/68) · CPC title
Microapparatus (sample containers with integrated microfluidic structures B01L3/5027) · CPC title
- C12Q1/6869Primary
Methods for sequencing · CPC title
- C07K14/245Primary
Escherichia (G) · CPC title
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Frequently asked questions
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- What does patent US12024541B2 cover?
- Provided is a method of characterising a polynucleotide using a transmembrane pore, wherein the pore is a double pore comprising a first Csg G pore, or a homologue thereof, and a second CsgG pore, or a homologue thereof.
- Who is the assignee on this patent?
- Oxford Nanopore Tech Plc, Vib Vzw, Univ Brussel Vrije
- What technology area does this patent fall under?
- Primary CPC classification C12Q1/6869. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jul 02 2024 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 12 related publications on this page (citations in our corpus or others sharing the same primary CPC).