Devices and methods for modifying optical properties

Patent metadata

Field	Value
Publication number	US-12023665-B2
Application number	US-201716081800-A
Country	US
Kind code	B2
Filing date	Mar 14, 2017
Priority date	Mar 14, 2016
Publication date	Jul 2, 2024
Grant date	Jul 2, 2024

Abstract

Devices and methods for modifying optical properties of biological samples or aspects thereof are provided. The subject methods include generating a reaction product with a device and reacting the reaction product to sufficiently modify an optical property to allow detection of the modified optical property.

First claim

The invention claimed is:

1. A biological sample assay optical property modifying device, the device comprising:
a) a sample receiving cartridge comprising i) a sample inlet, ii) reaction chambers each comprising an optical property modifying reagent, and iii) one or more conduits operatively connecting the reaction chambers with the sample inlet;
b) a substrate comprising:
i) a heating element; and
ii) a power source operatively coupled to the heating element; and

c) an adhesive layer operatively connecting the sample receiving cartridge and the substrate, wherein the adhesive layer forms a wall of each of the reaction chambers, and wherein the adhesive layer comprises one or more adhesive.

2. The device according to claim 1 , wherein each of the reaction chambers are microfluidic reaction chambers.

3. The device according to claim 1 , further comprising a selective venting element forming a wall of each of the reaction chambers.

4. The device according to claim 3 , wherein the selective venting element comprises a porous polymer matrix and a hydrogel having a passively tunable porosity.

5. The device according to claim 4 , wherein the porous polymer matrix comprises polyethylene.

6. The device according to claim 4 , wherein the hydrogel comprises carboxymethyl cellulose.

7. The device according to claim 1 , wherein each of the reaction chambers comprises a first opening on a first side of the sample receiving cartridge and a second opening on a second side of the sample receiving cartridge, wherein the first side is opposite the second side and the adhesive layer seals each second opening.

8. The device according to claim 7 , further comprising a selective venting element which seals each first opening.

9. The device according to claim 1 , wherein the substrate is a printed circuit board.

10. The device according to claim 1 , wherein the power source is a battery.

11. The device according to claim 1 , wherein the substrate comprises a control unit.

12. The device according to claim 11 , wherein the substrate comprises a sensor.

13. The device according to claim 12 , wherein the control unit activates the heating element to heat a sample in the reaction chambers when the sensor detects the sample.

14. The device according to claim 12 , wherein the substrate comprises a light source that emits light when the sensor detects the sample.

15. The device according to claim 11 , wherein the control unit is configured to perform a colorimetric analysis of a sample in the reaction chambers.

16. The device according to claim 1 , further comprising a housing.

17. The device according to claim 16 , wherein the housing comprises a first portion and a second portion, wherein the second portion is mateable with the first portion to encapsulate the sample receiving cartridge, the substrate and the adhesive layer.

18. The device according to claim 16 , wherein the housing has a volume of 300 cm 3 or less.

19. The device according to claim 1 , wherein the device is a hand-held device.

20. The device according to claim 1 , wherein the sample receiving cartridge is transparent.

21. The device according to claim 1 , wherein the sample receiving cartridge comprises a polymeric material.

22. The device according to claim 21 , wherein the polymeric material is polyethylene.

23. The device according to claim 1 , wherein the adhesive layer is transparent.

24. The device according to claim 1 , wherein the adhesive layer is reflective.

25. The device according to claim 1 , wherein the adhesive layer comprises an acrylic adhesive.

26. The device according to claim 1 , wherein the chambers each comprise a nucleic acid amplification composition.

27. The device according to claim 1 , wherein the biological sample is a nucleic acid amplification sample.

28. The device according to claim 1 , wherein the optical property modifying reagent is a halochromic reagent.

29. The device according to claim 1 , wherein the adhesive layer has a thermal conductivity of conductivity of 0.1 W/m-K to 10 W/m-K.

30. The device according to claim 1 , wherein the adhesive layer does not comprise an acid.

31. The device according to claim 1 , wherein the adhesive layer is opaque and white.

32. The device according to claim 1 , wherein the adhesive layer comprises a first layer laminated with a second layer.

33. The device according to claim 32 , wherein the first layer does not comprise an acid.

34. The device according to claim 32 , wherein the second layer is opaque and white.

35. The device according to claim 1 , wherein the optical property modifying reagent is an enzyme-linked immunosorbent assay (ELISA) reagent.

36. The device according to claim 35 , wherein the ELISA reagent is selected from the group consisting of alkaline phosphatase, horseradish peroxidase, β-galactosidase, BCIP/NBT (5-bromo-4-chloro-3-indolyl-phosphate/nitrobluetetrazolium), TMB (3,3′, 5,5′ tetramethylbenzidine), DAB (3,3′, 4,4′ diaminobenzidine), 4CN (4-chloro-1-naphthol), TMB (dual function substrate), ABTS (2,2′-azino-di [3-ethylbenzthiazoline] sulfonate), OPD (o-phenylenediamine), MUG (4-methylumbelliferyl galactoside), HPA (hydroxyphenylacetic acid), and HPPA (3-p-hydroxyphenylproprionic acid).

37. The device according to claim 1 , wherein the heating element comprises two or more heat-generating reactants that produce heat when mixed with one another.

38. The device according to claim 1 , wherein the adhesive layer is opaque and a color complementary to a reaction start color.

39. The device according to claim 1 , wherein the device further comprises a housing, the housing comprising:
a first portion and
a second portion mated with the first portion,
wherein the housing encapsulates the sample receiving cartridge, the substrate, and the adhesive layer and wherein the housing further comprises one or more inlet opening providing access to the sample inlet for a sample to be loaded into the sample receiving cartridge.

40. A method of modifying an optical property in a biological sample assay, the method comprising:
a. transmitting a biological sample into the reaction chambers of the biological sample assay optical property modifying device of claim 1 , thereby generating a reaction mixture;
b. heating the reaction mixture with a heating element of a substrate of the device and thereby generating a reaction product, wherein the substrate further comprises a power source operatively coupled to the heating element; and
c. reacting the reaction product with the optical property modifying reagent, wherein the reacting sufficiently modifies an optical property of the optical property modifying reagent to allow detection of the modified optical property.

41. The method according to claim 40 , wherein the biological sample comprises a nucleic acid.

42. The method according to claim 41 , wherein the reaction chambers further comprise an amplification composition.

43. The method according to claim 42 , wherein the heating accelerates a nucleic acid amplification reaction comprising the nucleic acid and the amplification com

Assignees

• Pfizer

Inventors

• Myers Iii Frank B
• Mitra Debkishore
• Waldeisen John Robert
• Dimov Ivan Krastev

Classifications

• B01L2300/168

  Specific optical properties, e.g. reflective coatings · CPC title

• B01L2300/123

  Flexible; Elastomeric · CPC title

• B01L2300/0627

  Sensor or part of a sensor is integrated · CPC title

• B01L2300/041

  Connecting closures to device or container · CPC title

• B01L2200/12

  Specific details about manufacturing devices · CPC title