Engineered DNA polymerase variants
US-11060075-B2 · Jul 13, 2021 · US
Engineered DNA polymerase variants
US12018295B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12018295-B2 |
| Application number | US-202117345404-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jun 11, 2021 |
| Priority date | Oct 29, 2018 |
| Publication date | Jun 25, 2024 |
| Grant date | Jun 25, 2024 |
How to read this patent
A practical reading order for non-experts. Skip the full description unless you need deep technical detail.
- Title
What the patent document calls the invention.
- Abstract
A short plain-language summary of the technical disclosure.
- Assignees and inventors
Who owns or filed the patent and who is credited as inventor.
- Key dates
Filing, priority, publication, and grant dates set the timeline.
- First independent claim
The legal scope of protection — read this for what is actually claimed.
- CPC / IPC classifications
Technology tags used to group this patent with similar filings.
- Citations and related patents
Prior art links and similar publications in this corpus.
Abstract
Official abstract text for this publication.
The present invention provides engineered DNA polymerase polypeptides and compositions thereof, as well as polynucleotides encoding the engineered DNA polymerase polypeptides. The invention also provides methods for use of the compositions comprising the engineered DNA polymerase polypeptides for diagnostic and other purposes.
First claim
Opening claim text (preview).
What is claimed is: 1. A polynucleotide comprising a polynucleotide sequence encoding an engineered DNA polymerase comprising a polypeptide sequence having at least 85% or more sequence identity to the reference sequence of SEQ ID NO: 6, wherein the polypeptide sequence comprises at least a substitution at amino acid position 515, wherein the amino acid positions are numbered with reference to SEQ ID NO: 6. 2. The polynucleotide of claim 1 , wherein said polynucleotide sequence encodes an engineered DNA polymerase comprising a polypeptide sequence having at least 0% or more sequence identity to the reference sequence of SEQ ID NO: 6. 3. The polynucleotide of claim 1 , wherein said polynucleotide sequence is operably linked to a control sequence. 4. The polynucleotide of claim 1 , wherein said polynucleotide sequence is codon-optimized. 5. An expression vector comprising a polynucleotide of claim 1 . 6. A host cell an expression vector of claim 5 . 7. A method of producing an engineered DNA polymerase polypeptide in a host cell, comprising culturing a host cell of claim 6 , under suitable culture conditions such that said engineered DNA polymerase is produced. 8. The method of claim 7 , further comprising recovering at least one engineered DNA polymerase from the culture and/or host cells. 9. The method of claim 8 , further comprising the step of purifying said engineered DNA polymerase. 10. The polynucleotide of claim 1 , wherein said polynucleotide sequence encodes an engineered DNA polymerase comprising a polypeptide sequence having at least 91%, 92%, 93%, or 94% or more sequence identity to the reference sequence of SEQ ID NO: 6. 11. The polynucleotide of claim 1 , wherein said polynucleotide sequence encodes an engineered DNA polymerase comprising a polypeptide sequence having at least 95%, 96%, 97%, or 98% or more sequence identity to the reference sequence of SEQ ID NO: 6. 12. The polynucleotide of claim 1 , wherein said polynucleotide sequence encodes an engineered DNA polymerase comprising a polypeptide sequence having at least 99% or more sequence identity to the reference sequence of SEQ ID NO: 6. 13. The polynucleotide of claim 1 , wherein the substitution at amino acid position 515 comprises substitution 515F, 515G, 515L, or 515R. 14. The polynucleotide of claim 1 , wherein the substitution at amino acid position 515 comprises substitution 515L.
Assignees
Inventors
Classifications
Genes encoding for enzymes or proenzymes · CPC title
DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase · CPC title
PCR · CPC title
fluorescence · CPC title
DNA polymerase · CPC title
Patent family
Related publications grouped by family.
External sources
Frequently asked questions
Answers are generated from the same data shown on this page.
- What does patent US12018295B2 cover?
- The present invention provides engineered DNA polymerase polypeptides and compositions thereof, as well as polynucleotides encoding the engineered DNA polymerase polypeptides. The invention also provides methods for use of the compositions comprising the engineered DNA polymerase polypeptides for diagnostic and other purposes.
- Who is the assignee on this patent?
- Codexis Inc
- What technology area does this patent fall under?
- Primary CPC classification C12N9/1252. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jun 25 2024 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 7 related publications on this page (citations in our corpus or others sharing the same primary CPC).