Method for increasing viridiflorol content in tissues

Patent metadata

Field	Value
Publication number	US-12018264-B2
Application number	US-202117226283-A
Country	US
Kind code	B2
Filing date	Apr 9, 2021
Priority date	Jun 24, 2020
Publication date	Jun 25, 2024
Grant date	Jun 25, 2024

Abstract

The present invention relates to the development of high viridiflorol containing variety for enhancing the aroma as well as medicinal properties of Mentha piperita by overexpressing MPTPS4 gene through genetic transformation. This transgenic plant possesses better growth and able to produce essential oil with good quality contains high viridiflorol maximum of about 25% and proportionate decrease in menthone, menthofuran and menthol of Mentha piperita as compared to other control genotype. Mentha piperita (CIM-madhuras) that produces viridiflorol, a molecule of potential demand in perfumery, cosmetics, toiletries, drugs and sanitation products.

First claim

We claim:

1. A method for expressing increasing viridiflorol content in Lamiaceae plant tissues, said method comprising the following steps:
a) isolating the MPTS4 gene of SEQ ID NO:4 from Mentha piperita;
b) cloning the MPTPS4 gene obtained at step (a) into a pHANNIBAL vector to generate the cassette of SEQ ID NO:8;
c) re-cloning the cassette obtained at step (b) into a pART27 binary vector system to obtain a vector comprising SEQ ID NO:9; and
d) transforming the vector obtained at step (c) into Agrobacterium tumefaciens , then further transfecting it into Lamiaceae plant tissues to produce Lamiaceae plant tissues exhibiting increased viridiflorol content compared to Lamiaceae plant tissues that are transfected with a control pART27 binary vector system that does not comprise the cassette of SEQ ID NO:8.

2. A transgenic plant tissue prepared by the method according to claim 1 .

3. A composition comprising the transgenic plant tissue according to claim 2 , wherein
the composition is a perfumery, a cosmetic, a toiletry, or a drug.

4. A composition comprising the transgenic plant tissue according to claim 2 , wherein the composition is a sanitation product.

Assignees

• Council Scient Ind Res
• Council Scient Ind Res

Inventors

• Shasany Ajit Kumar
• Yadav Pallavi
• Rastogi Shubhra
• Jalil Syed Uzma
• Bhakuni Rajendra Singh

Classifications

• C12N9/88

  Lyases (4.) · CPC title

• C12N15/8243 Primary

  involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine · CPC title

• C11B9/025 Primary

  Recovery by solvent extraction · CPC title