Detergent composition

Patent metadata

Field	Value
Publication number	US-12018236-B2
Application number	US-202117544026-A
Country	US
Kind code	B2
Filing date	Dec 7, 2021
Priority date	Apr 11, 2014
Publication date	Jun 25, 2024
Grant date	Jun 25, 2024

Abstract

The present invention concerns a detergent and a pharmaceutical composition comprising a deoxyribonuclease (DNase), wherein the DNase is obtained from a fungal source. It further concerns a laundering method and the use of DNase. The present invention further relates to polypeptides having DNase activity, nucleotides encoding the polypeptide, as well as methods of producing the polypeptides.

First claim

The invention claimed is:

1. A nucleic acid construct or expression vector comprising a polynucleotide encoding a polypeptide having DNase activity, wherein the polypeptide has at least 85% sequence identity to the polypeptide of SEQ ID NO: 2, SEQ ID NO: 8, or SEQ ID NO: 9, or is a fragment thereof possessing DNase activity, operably linked to one or more control sequences that direct the production of the polypeptide in a recombinant host cell.

2. A recombinant host cell comprising the nucleic acid construct or expression vector of claim 1 .

3. A method of producing a polypeptide having DNase activity, comprising cultivating a recombinant host cell of claim 2 under conditions conducive for production of the polypeptide.

4. The method of claim 3 , further comprising recovering the polypeptide.

5. The method of claim 3 , wherein the polypeptide is foreign to the recombinant host cell.

6. The method of claim 3 , wherein the polynucleotide is foreign to the recombinant host cell.

7. The method of claim 3 , wherein the recombinant host cell is a filamentous fungal host cell selected from the group consisting of Acremonium, Aspergillus, Aureobasidium, Bjerkandera, Ceriporiopsis, Chrysosporium, Coprinus, Coriolus, Cryptococcus, Filibasidium, Fusarium, Humicola, Magnaporthe, Mucor, Myceliophthora, Neocallimastix, Neurospora, Paecilomyces, Penicillium, Phanerochaete, Phlebia, Piromyces, Pleurotus, Schizophyllum, Talaromyces, Thermoascus, Thielavia, Tolypocladium, Trametes , and Trichoderma cells.

8. The method of claim 7 , wherein the polypeptide has at least 90% sequence identity to the polypeptide of SEQ ID NO: 2, SEQ ID NO: 8, or SEQ ID NO: 9.

9. The method of claim 7 , wherein the polypeptide has at least 95% sequence identity to the polypeptide of SEQ ID NO: 2, SEQ ID NO: 8, or SEQ ID NO: 9.

10. The method of claim 7 , wherein the polypeptide is a fragment of SEQ ID NO: 2, SEQ ID NO: 8, or SEQ ID NO: 9 and the polypeptide has DNase activity.

11. A method for laundering a textile, comprising:
a) exposing the textile to a wash liquor comprising a detergent composition comprising:
(i) a polypeptide having DNase activity, wherein the polypeptide has at least 85% sequence identity to the polypeptide of SEQ ID NO: 2, SEQ ID NO: 8, or SEQ ID NO: 9, or a fragment thereof possessing DNase activity, and
(ii) a surfactant,

wherein the detergent composition is formulated as a bar, a homogenous tablet, a tablet having two or more layers, a pouch having one or more compartments, a powder, a granule, a paste, a gel, or a liquid; and
b) completing at least one wash cycle.

12. The method of claim 11 , further comprising rinsing the textile.

13. The method of claim 11 , wherein the composition further comprises one or more additional enzymes.

14. The method of claim 13 , wherein the one or more additional enzymes are selected from the group consisting of amylases, arabinases, carbohydrases, cellulases, cutinases, galactanases, lipases, mannanases, oxidases, pectinases, proteases, and xylanases.

15. The method of claim 14 , wherein the composition comprises a protease.

16. The method of claim 11 , wherein the composition further comprises a detergent adjunct ingredient selected from the group consisting of builders, flocculating aid, chelating agents, dye transfer inhibitors, enzymes, enzyme stabilizers, enzyme inhibitors, catalytic materials, bleach activators, hydrogen peroxide, sources of hydrogen peroxide, preformed peracids, polymeric dispersing agents, clay soil removal/anti-redeposition agents, brighteners, suds suppressors, dyes, perfumes, structure elasticizing agents, fabric softeners, carriers, hydrotropes, co-builders, fabric hueing agents, anti-foaming agents, dispersants, processing aids, and/or pigments.

17. The method of claim 11 , wherein the polypeptide has at least 90% sequence identity to the polypeptide of SEQ ID NO: 2, SEQ ID NO: 8, or SEQ ID NO: 9.

18. The method of claim 11 , wherein the polypeptide has at least 95% sequence identity to the polypeptide of SEQ ID NO: 2, SEQ ID NO: 8, or SEQ ID NO: 9.

19. The method of claim 11 , wherein the polypeptide is a fragment of SEQ ID NO: 2, SEQ ID NO: 8, or SEQ ID NO: 9 and the polypeptide has DNase activity.

Assignees

• Novozymes As

Inventors

• Gori Klaus
• Allesen-Holm Marie
• Baltsen Lilian Eva Tang
• Noergaard Allan
• Lehmbeck Jan

Classifications

• C12Y301/22002

  Aspergillus deoxyribonuclease K(1) (3.1.22.2) · CPC title

• C12N15/52

  Genes encoding for enzymes or proenzymes · CPC title

• C12N9/22

  Ribonucleases {[RNase]; Deoxyribonucleases [DNase]} · CPC title

• C11D3/0068 Primary

  Deodorant compositions · CPC title

• C11D2111/12

  Soft surfaces, e.g. textile · CPC title