Process for extracting substances of interest

Patent metadata

Field	Value
Publication number	US-12016957-B2
Application number	US-201917297530-A
Country	US
Kind code	B2
Filing date	Nov 27, 2019
Priority date	Nov 27, 2018
Publication date	Jun 25, 2024
Grant date	Jun 25, 2024

Abstract

The present description relates to a method for extracting at least one substance of interest, the method comprising the following steps: —providing an admixture comprising water, a source comprising the at least one substance of interest or at least one precursor thereof, at least one surfactant and at least one lipophilic compound; —the emulsification of the admixture, leading to the formation of an emulsion; wherein the emulsion comprises: —a continuous aqueous phase; and a discontinuous lipidic phase comprising the at least one lipophilic compound, the at least one surfactant and at least one substance of interest extracted from the source during the emulsification; wherein the method further comprises the addition of at least one cryoprotectant to the admixture and the lyophilization of the emulsion.

First claim

The invention claimed is:

1. A process for extracting at least one substance of interest from a source, the process comprising the following steps of:
providing a mixture comprising water, at least one surfactant, at least one lipophilic compound, and a source, the source comprising the at least one substance of interest or at least one precursor thereof; and
emulsifying the mixture, thereby forming an emulsion and extracting the at least one substance of interest from the source;
wherein the emulsion comprises:
a continuous aqueous phase; and
a discontinuous lipid phase comprising the at least one lipophilic compound, the at least one surfactant, and the at least one substance of interest extracted from the source during emulsifying;
wherein the source is a plant source containing at least a portion of at least one plant, an animal source containing at least one part of at least one animal, a mineral source, a prokaryote source, and/or a unicellular eukaryote source; and

wherein the process further comprises adding at least one cryoprotectant to the mixture and freeze-drying the emulsion; and
wherein adding at least one cryoprotectant to the mixture is performed after emulsifying the mixture, in a weight-to-volume ratio of at least 5% w/v based on the total volume of the mixture excluding the source.

2. The process of claim 1 , wherein emulsifying the mixture comprises sonicating the mixture.

3. The process of claim 1 , wherein the source is a plant source containing at least a portion of at least one plant.

4. The process of claim 1 , wherein the at least one surfactant is selected from the group consisting of Dendri-TAC type dendrimers, F i TAC n or F i TAC n type oligomers, TPGS 1000, TPGS 750M, sugar- and/or amino acid-derived surfactants, and combinations thereof.

5. The process of claim 1 , wherein emulsifying is performed by maintaining the temperature of the mixture between about 4° C. and about 60° C.

6. The process of claim 1 , wherein the duration of emulsifying is comprised between about 0.5 seconds and about 5 hours.

7. The process of claim 1 , wherein said discontinuous lipid phase is in the form of droplets of a predetermined mean diameter.

8. The process of claim 1 , wherein the at least one cryoprotectant is selected from the group consisting of polymers, amino acids, saccharide compounds, and combinations thereof.

9. The process of claim 1 , wherein the at least one lipophilic compound, the at least one surfactant, and the at least one cryoprotectant are biocompatible.

10. The process of claim 1 , further comprising a step of pretreating prior to emulsifying the mixture, wherein the step of pretreating comprises macerating the mixture at a temperature between about 20° C. and about 60° C.

Assignees

• Avignon Univ
• Lyofal
• Centre Nat Rech Scient
• Enscm
• Univ Montpellier

Inventors

• Contino-Pepin Christiane
• Desgranges Stéphane
• Dall'Armellina Alice
• Duval Charles
• Letan-Martin Mathias

Classifications

• B01D11/0265

  Applying ultrasound · CPC title

• A61K2236/51

  Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying · CPC title

• A61K2236/35

  Extraction with lipophilic solvents, e.g. Hexane or petrol ether · CPC title

• A61K2236/331

  using water, e.g. cold water, infusion, tea, steam distillation or decoction · CPC title

• A61K47/44

  Oils, fats or waxes according to two or more groups of A61K47/02-A61K47/42; Natural or modified natural oils, fats or waxes, e.g. castor oil, polyethoxylated castor oil, montan wax, lignite, shellac, rosin, beeswax or lanolin (synthetic glycerides, e.g. medium-chain triglycerides, A61K47/14) · CPC title