Compositions comprising recombinant Bacillus cells and an insecticide

Patent metadata

Field	Value
Publication number	US-12016912-B2
Application number	US-202117356958-A
Country	US
Kind code	B2
Filing date	Jun 24, 2021
Priority date	Sep 17, 2014
Publication date	Jun 25, 2024
Grant date	Jun 25, 2024

Abstract

The present invention relates to a composition comprising a) recombinant exosporium-producing Bacillus cells that express a fusion protein comprising: (i) at least one plant growth stimulating protein or peptide and (ii) a targeting sequence that localizes the fusion protein to the exosporium of the Bacillus cells; and b) at least one particular insecticide disclosed herein in a synergistically effective amount. Furthermore, the present invention relates to the use of this composition as well as a method for enhancing plant growth, promoting plant health, and/or reducing overall damage of plants and plant parts.

First claim

What is claimed is:

1. A composition comprising:
a) recombinant exosporium-producing Bacillus cells of a Bacillus cereus family member that express a fusion protein comprising:
(i) an endoglucanase sequence having at least 95% sequence identity to SEQ ID NO: 107; and
(ii) a targeting sequence comprises:
an amino acid sequence having at least 43% identity with amino acids 20-35 of SEQ ID NO: 1, wherein the identity with amino acids 25-35 is at least 54%;
a targeting sequence comprising amino acids 1-35 of SEQ ID NO: 1;
a targeting sequence comprising amino acids 20-35 of SEQ ID NO: 1;
a targeting sequence comprising amino acids 22-31 of SEQ ID NO: 1;
a targeting sequence comprising amino acids 22-33 of SEQ ID NO: 1;
a targeting sequence comprising amino acids 20-31 of SEQ ID NO: 1; or
a targeting sequence comprising SEQ ID NO: 1; and

b) abamectin;
in a synergistically effective amount.

2. The composition according of claim 1 , wherein the Bacillus cereus family member is selected from the group consisting of Bacillus anthracis, Bacillus cereus, Bacillus thuringiensis, Bacillus mycoides, Bacillus pseudomycoides, Bacillus samanii, Bacillus gaemokensis, Bacillus weihenstephensis, Bacillus toyoiensis and combinations thereof.

3. The composition of claim 1 , wherein the endoglucanase sequence in the fusion protein comprises SEQ ID NO: 107.

4. The composition according to claim 1 , wherein the fusion protein is expressed under the control of a sporulation promoter native to the targeting sequence of the fusion protein.

5. The composition according to claim 4 , wherein the fusion protein is expressed under the control of a high-expression sporulation promoter.

6. The composition according to claim 4 , wherein the sporulation promoter comprises a nucleic acid sequence having at least 95% identity with a nucleic acid sequence of any one of SEQ ID NOs: 85-103.

7. A seed coated with the composition of claim 1 .

Assignees

• Bayer Cropscience Lp
• Spogen Biotech Inc

Inventors

• Curtis Damian
• Thompson Brian

Classifications

• C12Y301/01004

  Phospholipase A2 (3.1.1.4) · CPC title

• C12Y302/01132

  Chitosanase (3.2.1.132) · CPC title

• C12Y302/01004

  Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase · CPC title

• C12Y301/01 Primary

  Carboxylic ester hydrolases (3.1.1) · CPC title

• A61K2035/115

  Probiotics · CPC title