Tissue catalyzed growth of polymer as epithelial linings for therapy

Patent metadata

Field	Value
Publication number	US-12016901-B2
Application number	US-202017118521-A
Country	US
Kind code	B2
Filing date	Dec 10, 2020
Priority date	Dec 13, 2019
Publication date	Jun 25, 2024
Grant date	Jun 25, 2024

Abstract

The present disclosure provides compositions, methods, and kits that enable the in situ growth of polymers on or within a subject. In some aspects, the monomer, dopamine, polymerizes in vivo to form a polymer on a tissue. In additional aspects, the compositions, methods, and kits are useful for treating or preventing a disease or disorder.

First claim

What is claimed is:

1. A method of forming a dopamine polymer on a surface of a tissue in a subject in need thereof, the method comprising administering to the subject a composition comprising a plurality of molecules of dopamine, or a salt thereof, and at least a minimum amount of an oxygen source to polymerize the dopamine on the surface of the tissue, wherein the administering step delivers the plurality of molecules of dopamine, or a salt thereof and the oxygen source to the tissue of the subject, allowing the oxygen source to contact a catalyst in the tissue of the subject, and wherein the oxygen source reacts with the catalyst in the tissue to release oxygen from the oxygen source, and wherein the oxygen released from the oxygen source polymerizes the plurality of molecules of dopamine, or a salt thereof on the surface of the tissue, thereby forming said dopamine polymer on the surface of said tissue; wherein:
the oxygen source is hydrogen peroxide or urea hydrogen peroxidalk; and the catalyst present in the tissue is catalase or peroxidase.

2. The method of claim 1 , wherein the peroxidase is eosinophil peroxidase, lactoperoxidase, or myeloperoxidase.

3. The method of claim 1 , wherein the catalyst is a catalase.

4. The method of claim 1 , wherein the catalyst is located in the gastrointestinal (GI) tract of the subject.

5. The method of claim 1 , wherein the composition further comprises an enzyme, a nutrient blocker, a nutraceutical, a radioprotective agent, an active pharmaceutical ingredient, a diagnostic agent, or a combination thereof.

6. The method of claim 1 , wherein the composition is administered orally to the subject.

7. The method of claim 1 , wherein the composition comprises about 0.01 to about 50 mg/mL of dopamine.

8. The method of claim 1 , wherein the composition comprises about 1 mM to about 30 mM of the oxygen source.

9. The method of claim 1 , wherein the composition has a pH of about 7 to about 10.

10. The method of claim 1 , wherein the tissue comprises an epithelium and the dopamine polymer forms in contact with the epithelium.

11. The method of claim 5 , wherein the composition further comprises an enzyme.

12. The method of claim 11 , wherein the enzyme is a digestive enzyme.

13. The method of claim 12 , wherein the digestive enzyme is lactase, peptidase, sucrase, maltase, amylase, a lipase, or a protease.

14. The method of claim 1 , wherein the composition further comprises an active pharmaceutical ingredient.

15. The method of claim 14 , wherein the active pharmaceutical ingredient is praziquantel.

Assignees

• Massachusetts Inst Technology
• Brigham & Womens Hospital Inc

Inventors

• Langer Robert S
• Traverso Carlo Giovanni
• Li Junwei
• Wang Thomas
• Kirtane Ameya R
• Shi Yunhua

Classifications

• A61L2400/04

  Materials for stopping bleeding · CPC title

• A61L31/16

  Biologically active materials, e.g. therapeutic substances {(A61L31/047 takes precedence)} · CPC title

• A61L31/06

  obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds {(A61L31/041 takes precedence)} · CPC title

• A61L31/048

  obtained by reactions only involving carbon-to-carbon unsaturated bonds (A61L31/041 takes precedence) · CPC title

• A61L31/042

  Polysaccharides · CPC title