Isolated Spodoptera Frugiperda Multiple Nucleopolyhedroviruses and Methods for Killing Insects
US-2016010062-A1 · Jan 14, 2016 · US
Insect toxin delivery mediated by a densovirus coat protein
US12016333B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12016333-B2 |
| Application number | US-202117470055-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 9, 2021 |
| Priority date | Sep 27, 2016 |
| Publication date | Jun 25, 2024 |
| Grant date | Jun 25, 2024 |
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- Title
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Abstract
Official abstract text for this publication.
A coat protein of Junonia coenia densovirus (JcDNV) is used to deliver attached peptide insect toxin across the gut epithelium of a fall armyworm, Spodoptera frugiperda. A fusion protein comprising VP4 attached to an insect toxin via a peptide linker is developed. A composition comprising a JcDNV coat protein attached to an insect toxin via a peptide linker can be used for insect pest control.
First claim
Opening claim text (preview).
What is claimed is: 1. A recombinant DNA comprising a nucleic acid sequence encoding a fusion protein, wherein the fusion protein comprises a carrier protein attached to an insect toxin via a peptide linker, wherein the carrier protein is a Junonia coenia densovirus (JcDNV) coat protein. 2. The recombinant DNA according to claim 1 , wherein the JcDNV coat protein is VP4 that has an amino acid sequence SEQ ID NO: 2. 3. The recombinant DNA according to claim 1 , wherein the insect toxin is Hv1a that has an amino acid sequence SEQ ID NO: 6. 4. An expression cassette for expression of a fusion protein comprising a regulatory region operably linked to the recombinant DNA of claim 1 . 5. A vector comprising the expression cassette of claim 4 . 6. The recombinant DNA of claim 1 , wherein the JcDNV coat protein is VP1 that has an amino acid sequence SEQ IN NO: 1. 7. The recombinant DNA of claim 1 , wherein the peptide linker is a protease-resistant linker. 8. The recombinant DNA of claim 1 , wherein the fusion protein comprises VP4 attached to the N-terminus of insect toxin Hv1Ia through a proline rich linker, and wherein the peptide linker has an amino acid sequence SEQ ID NO: 4. 9. The recombinant DNA of claim 1 , wherein the insect toxin comprises an arthropod-derived insecticidal peptide. 10. The recombinant DNA of claim 1 , wherein the insect toxin is an Androctonus australis Hector insect toxin (AaHIT) or a Scorpion Leiurus quinquestriatus hebraeus toxin.
Assignees
Inventors
Classifications
expressing industrially exogenous proteins, e.g. for pharmaceutical use, human insulin, blood factors, immunoglobulins, pseudoparticles · CPC title
Viral vectors · CPC title
Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00) · CPC title
Parvoviridae, e.g. feline panleukopenia virus, human parvovirus · CPC title
Aerosols · CPC title
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- What does patent US12016333B2 cover?
- A coat protein of Junonia coenia densovirus (JcDNV) is used to deliver attached peptide insect toxin across the gut epithelium of a fall armyworm, Spodoptera frugiperda. A fusion protein comprising VP4 attached to an insect toxin via a peptide linker is developed. A composition comprising a JcDNV coat protein attached to an insect toxin via a peptide linker can be used for insect pest control.
- Who is the assignee on this patent?
- Univ Florida, Univ Iowa State Res Found Inc, Univ Florida
- What technology area does this patent fall under?
- Primary CPC classification A01N37/46. Mapped technology areas include Human Necessities.
- When was this patent published?
- Publication date Tue Jun 25 2024 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 1 related publication on this page (citations in our corpus or others sharing the same primary CPC).