Raman-active nanoparticles and method of preparing the same

Patent metadata

Field	Value
Publication number	US-12013393-B2
Application number	US-201816498326-A
Country	US
Kind code	B2
Filing date	Oct 10, 2018
Priority date	Oct 8, 2018
Publication date	Jun 18, 2024
Grant date	Jun 18, 2024

Abstract

Provided is a method of preparing Raman-active nanoparticles, which includes a) preparing a metal nanocore having a nano-star shape from a first reaction solution in which a first metal precursor is mixed with a buffer solution; b) fixing a Raman reporter in the metal nanocore; and c) forming a metal shell, which surrounds the nanocore in which the Raman reporter is fixed, from a second reaction solution in which a second metal precursor is mixed with the nanocore in which the Raman reporter is fixed. The Raman reporter has a binding affinity for each of a first metal of the metal nanocore and a second metal of the metal shell.

First claim

The invention claimed is:

1. A method of preparing Raman-active nanoparticles, the method comprising:
a) preparing a gold nanocore having a nano-star shape from a first reaction solution in which a first gold precursor is mixed with only a 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer solution;
b) fixing a 1,4-benzenedithiol (BDT) to the gold nanocore after a) is performed; and
c) forming a gold metal shell, which surrounds the nanocore in which the BDT is fixed, from a second reaction solution in which a second gold precursor is mixed with the nanocore in which the BDT is fixed after b) is performed,
wherein a size and/or shape of the nanocore is adjusted by controlling a factor of a molar ratio of the HEPES of the buffer solution to the first gold precursor in a) to be about 700 and controlling the pH to be about 7.2, such that the resulting Raman-active nanoparticles elicit Raman signals coincident with innate surface-enhanced Raman scattering (SERS) signals of the BDT,
wherein the BDT has a binding affinity for each of the gold nanocore and the gold metal shell.

2. The method of claim 1 , wherein a self-assembled monolayer of the BDT surrounding the metal nanocore is formed in the fixing of the Raman reporter in the metal nanocore.

3. The method of claim 1 , wherein the factor further comprises a pH of the buffer solution.

4. The method of claim 1 , wherein the second reaction solution comprises a surfactant, or comprises a surfactant and an organic acid.

5. The method of claim 1 , further comprising, after the forming of the metal shell from the second reaction solution:
d) fixing a receptor, which binds to an analyte, in the metal shell.

Assignees

• Korea Res Inst Standards & Sci

Inventors

• You Eun-Ah
• Kim Wansun
• Lee Tae Geol

Classifications

• G01N33/553 Primary

  Metal or metal coated · CPC title

• G01N21/658

  enhancement Raman, e.g. surface plasmons · CPC title

• B82Y40/00

  Manufacture or treatment of nanostructures · CPC title

• B82Y15/00

  Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors · CPC title

• B82Y5/00

  Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery · CPC title