Capillary electrophoresis apparatus

Patent metadata

Field	Value
Publication number	US-12013369-B2
Application number	US-202117406686-A
Country	US
Kind code	B2
Filing date	Aug 19, 2021
Priority date	Mar 18, 2016
Publication date	Jun 18, 2024
Grant date	Jun 18, 2024

Abstract

A capillary electrophoresis apparatus is disclosed that does not discharge and achieves both compactness and performance even with a part configuration having insufficient spatial distance or creeping distance. This capillary electrophoresis apparatus is provided with a resistance heater for heating capillaries, an electrode holder that holds capillary electrodes and is connected to a high-voltage unit, and a conductive member that at least partially comprises metal and has been grounded to a low potential. The electrode holder and conductive member are in contact with heat-dissipating rubber disposed there between that composes a structure comprising an insulation member. As a result of this configuration, discharge risk is reduced through the reduction of the potential of parts near the high-voltage unit and the slow reduction of the high potential of the high-voltage unit.

First claim

The invention claimed is:

1. A capillary electrophoresis apparatus for analyzing a sample by electrophoresis using a capillary, the apparatus comprising:
a heater being a heat source;
a conductive member at least part of which is made of metal;
a thermostatic bath base made of a first insulating member to which the heater and the conductive member are attached;
a high voltage portion to which an electrode plug is connected; and
a thermal insulation material that is in contact with the thermostatic bath base and is located between the thermostatic bath base and the conductive member, wherein
the conductive member is in contact with a ground site, and is applied with an insulating treatment comprising the thermal insulation material,
the ground site is at least one of an earth plate, earth wire, frame ground, or frame of the apparatus, such that the ground site is grounded to the conductive member while avoiding the surfaces of the thermal insulation material and the heater,
a thickness of the thermal insulation material varies in proportion to a distance from the high voltage portion,
the conductive member is subjected to a stepwise voltage according to the distance from the high voltage portion, after the insulating treatment,
a lower side of the conductive member is in contact with the thermostatic bath base,
the thermostatic bath base is located between the conductive member and the high voltage portion, and
the capillary electrophoresis apparatus has a second structure made of a third insulating member between the conductive member and the capillary.

2. The capillary electrophoresis apparatus according to claim 1 ,
wherein the conductive member and the high voltage portion are in contact with each other through a first structure composed of a single or a plurality of layers and made of a second insulating member.

3. The capillary electrophoresis apparatus according to claim 1 ,
wherein the conductive member is applied with a chamfer.

4. The capillary electrophoresis apparatus according to claim 3 ,
wherein the conductive member and the high voltage portion are in contact with each other through a first structure composed of a single or a plurality of layers and a second insulating member.

5. The capillary electrophoresis apparatus according to claim 1 ,
wherein the second structure is a heat dissipation rubber.

6. The capillary electrophoresis apparatus according to claim 5 ,
wherein the conductive member and the high voltage portion are in contact with each other through a first structure composed of a single or a plurality of layers and a second insulating member.

7. The capillary electrophoresis apparatus according to claim 5 ,
wherein the conductive member is applied with a chamfer.

8. The capillary electrophoresis apparatus according to claim 7 ,
wherein the conductive member and the high voltage portion are in contact with each other through a first structure composed of a single or a plurality of layers and a second insulating member.

9. The capillary electrophoresis apparatus according to claim 1 ,
wherein the conductive member is a metal plate having a thickness of 1.0-10 mm.

Assignees

• Hitachi High Tech Corp

Inventors

• Nakazawa Taro
• Yamazaki Motohiro
• Uchida Kenko
• Fujii Akira

Classifications

• G01N27/44704

  Details; Accessories · CPC title

• G01N27/44713 Primary

  Particularly adapted electric power supply · CPC title

• G01N27/44791 Primary

  Microapparatus (sample containers with integrated microfluidic structures B01L3/5027) · CPC title

• G01N27/447

  using electrophoresis · CPC title

• G01N27/44708

  Cooling · CPC title