Microfluidic device

Patent metadata

Field	Value
Publication number	US-12005439-B2
Application number	US-202016833247-A
Country	US
Kind code	B2
Filing date	Mar 27, 2020
Priority date	Mar 29, 2019
Publication date	Jun 11, 2024
Grant date	Jun 11, 2024

Abstract

A microfluidic device for thermocycling of a reaction mixture is provided. The device comprises an inlet opening, an outlet opening, a flow channel connecting the inlet opening and the outlet opening and defining a flow direction from the inlet opening through the flow channel to the outlet opening, wherein the flow channel comprises a first flow channel surface and a second flow channel surface opposite to the first flow channel surface, and an array of wells provided in the first flow channel surface for fluidic communication with the inlet opening and the outlet opening. Further, the first flow channel surface provides a first hydrophilicity and at least a part of the second flow channel surface provides a second hydrophilicity, wherein the first hydrophilicity is greater than the second hydrophilicity.

First claim

The invention claimed is:

1. A microfluidic device for thermocycling of a reaction mixture, comprising:
an inlet opening;
an outlet opening;
a flow channel connecting said inlet opening and said outlet opening and defining a flow direction from the inlet opening through the flow channel to the outlet opening, wherein the flow channel comprises a first flow channel surface;
a second flow channel surface opposite to the first flow channel surface;
and an array of wells provided in said first flow channel surface for fluidic communication with the inlet opening and the outlet opening, wherein each well of the wells of the array of wells has a well shape in the first flow channel surface in a form of a regular hexagon elongated in the flow direction such that a length of the well from a first corner to a second opposite corner in the flow direction is greater than a width of the well from a first flat to a second parallel flat wherein the width is perpendicular to the flow direction, wherein the first flow channel surface comprises a first hydrophilicity having a surface contact angle in a range of 30° to 50° and at least a part of the second flow channel surface comprises a second hydrophilicity having a surface contact angle in a range of 80° to 90°, such that said first hydrophilicity is greater than said second hydrophilicity.

2. The microfluidic device of claim 1 , wherein the first hydrophilicity or the second hydrophilicity or both is provided by at least one of a surface treatment and a hydrophilic coating.

3. The microfluidic device of claim 1 , wherein a vertex of each well is oriented in the flow direction facing towards the inlet opening, wherein two vertexes of each well arranged opposite to each other are oriented in parallel to the flow direction.

4. The microfluidic device of claim 1 , wherein each well comprises a well length in the flow direction in a range of 50 μm to 300 μm, a well width perpendicular to the well length in a range of 25 μm to 150 μm, or a well depth in a range of 25 μm to 200 μm.

5. The microfluidic device of claim 1 , wherein each well comprises a rounded edge.

6. The microfluidic device of claim 5 , wherein the rounded well edge is rounded by a radius of less than 10 μm.

7. The microfluidic device of claim 1 , wherein a rim is provided between adjacent wells for fluidic separation of the adjacent wells, each rim comprising a width greater than 10 μm.

8. The microfluidic device of claim 1 , wherein an aspect ratio between a height of the flow channel and a length of each well is in a range between 0.3 and 0.7.

9. The microfluidic device of claim 1 , wherein a height of the flow channel is in a range of 25 μm to 200 μm.

10. The microfluidic device of claim 1 , wherein the microfluidic device consists of two parts attached detachably along a longitudinal axis of the microfluidic device.

11. The microfluidic device according to claim 10 , wherein the flow channel with the array of wells, the inlet opening and the outlet opening are disposed in a first part of the microfluidic device wherein the first part comprises the first flow channel surface, and wherein a cover part is disposed in a second part of the microfluidic device wherein the second part comprises the second flow channel surface.

12. The microfluidic device of claim 1 , wherein the microfluidic device is for digital PCR or biochemical assaying of a reaction mixture sample wherein the microfluidic device is configured to receive the reaction mixture in each of the wells via said flow channel.

13. The microfluidic device of claim 1 , wherein the microfluidic device is a consumable and comprises a transparent material comprising at least one of Cyclic Olefin Copolymer COC and Cyclic Olephin Polymer COP.

Assignees

• Roche Molecular Systems Inc

Inventors

• Meier Alexander
• Steinert Chris
• Zeder Michael

Classifications

• B01L2300/18

  Means for temperature control · CPC title

• B01L2300/168

  Specific optical properties, e.g. reflective coatings · CPC title

• B01L2300/161

  Control and use of surface tension forces, e.g. hydrophobic, hydrophilic · CPC title

• B01L2300/12

  Specific details about materials · CPC title

• B01L2300/0816

  Cards, e.g. flat sample carriers usually with flow in two horizontal directions · CPC title