Microfluidic thromboelastometry instrument

Patent metadata

Field	Value
Publication number	US-11998911-B2
Application number	US-201817260822-A
Country	US
Kind code	B2
Filing date	Jul 29, 2018
Priority date	Jul 29, 2018
Publication date	Jun 4, 2024
Grant date	Jun 4, 2024

Abstract

A microfluid thromboelastometry instrument for analyzing a viscosity of a sample fluid is provided. The microfluid thromboelastometry instrument includes a cartridge for measuring changes in the viscosity of a fluid, the cartridge includes an inlet and an outlet, wherein a flow of the fluid is provided through the cartridge between the inlet and the outlet. The cartridge includes at least one micro-pillar having at least one first position, the at least one micro-pillar is capable of bending with a fluid flow, and an amount of bending increases as the viscosity of the fluid increasing and restores to the at least one first position of the at least one micro-pillars in response to viscosity decrease of the fluid. The micro-pillar is fixed to the surface of the cartridge from the root and has a free distal extremity. A displacement of the micro-pillar distal extremity relative to the micro-pillar root is monitored.

First claim

What is claimed is:

1. A cartridge for measuring changes in a viscosity of a fluid, comprising an inlet and an outlet, wherein a flow of the fluid is provided through the cartridge between the inlet and the outlet, wherein
the cartridge comprises at least one micro-pillar having at least one first position, the at least one micro-pillar is configured to bend with increasing of the flow and the viscosity of the fluid and to restore to the at least one first position of the at least one micro-pillar in response to decreasing of the viscosity of the fluid,
the at least one micro-pillar is fixed to a surface of the cartridge from a root and the at least one micro-pillar has a free distal extremity,
a displacement of the free distal extremity of the at least one micro-pillar relative to the root of the at least one micro-pillar is monitored.

2. The cartridge according to claim 1 , wherein a plurality of micro-pillars forms a micro-pillar matrix, and the micro-pillar matrix covers the cartridge in a transverse direction in areas where the plurality of micro-pillars exist.

3. The cartridge according to claim 1 , wherein a plurality of micro-pillars are positionally configured to create channel structures between the plurality of micro-pillars for a blood sample injected into the cartridge to move back and forth in microfluidic channels.

4. The cartridge according to claim 3 , wherein the plurality of micro-pillars are equidistant from each other.

5. The cartridge according to claim 3 , wherein the microfluidic channels provide a linear path between the inlet and the outlet.

6. The cartridge according to claim 3 , wherein the microfluidic channels provide a non-linear path between the inlet and the outlet.

7. The cartridge according to claim 2 , wherein the micro-pillar matrix comprises a plurality of mixing protrusions to operate as mixing bodies for chemicals and the fluid.

8. The cartridge according to claim 2 , wherein the micro-pillar matrix comprises a plurality of mixing protrusions to operate as a flow rate regulator.

9. The cartridge according to claim 2 , wherein the micro-pillar matrix comprises the plurality of micro-pillars to be monitored for a viscosity change analysis.

10. The cartridge according to claim 2 , wherein the micro-pillar matrix comprises a first micro-pillar field providing homogenization as well as introducing the fluid to the cartridge.

11. The cartridge according to claim 2 , wherein the micro-pillar matrix comprises a second micro-pillar field, wherein a primary objective of the micro-pillar matrix is configured to provide data for a viscosity analysis through monitoring the displacement of the free distal extremity of the at least one micro-pillar.

12. The cartridge according to claim 11 , wherein the micro-pillar matrix comprises a third micro-pillar field regulating a blood flow rate and a chemical mixing process to ensure an accuracy of a monitoring process at the second micro-pillar field.

13. A thromboelastography instrument, comprising the cartridge according to claim 1 and an analysing structure, wherein the analyzing structure comprises a pumping unit, wherein the pumping unit accelerates a viscosity change stage by moving the fluid injected into the cartridge back and forth through microfluidic channels.

14. The thromboelastography instrument according to claim 13 , wherein the analyzing structure comprises an imaging unit, wherein the imaging unit monitors the at least one micro-pillar to detect the displacement of the free distal extremity of the at least one micro-pillar relative to the root of the at least one micro-pillar.

15. The thromboelastography instrument according to claim 14 , wherein the imaging unit monitors a micro-pillar cluster in a second micro-pillar field to detect the displacement of the free distal extremity of the at least one micro-pillar relative to the root of the at least one micro-pillar.

16. The thromboelastography instrument according to claim 14 , wherein the imaging unit comprises a high speed camera to observe a displacement of the at least one micro-pillar.

17. The thromboelastography instrument according to claim 14 , wherein the imaging unit increases an optical visibility of the at least one micro-pillar in an adverse circumstance by activating an extra light source.

18. The thromboelastography instrument according to claim 13 , wherein the analyzing structure comprises heating units to provide constant temperature values for the fluid in the cartridge.

19. The thromboelastography instrument according to claim 18 , wherein the heating units are positioned on a top surface of the cartridge and a bottom surface of the cartridge.

20. The thromboelastography instrument according to claim 18 , wherein the heating units extend along the microfluidic channels.

21. The thromboelastography instrument according to claim 18 , wherein the heating units are set to automatically heat up the fluid inside the cartridge to 37° C.

22. The thromboelastography instrument according to claim 13 , wherein the analyzing structure comprises a light source, wherein a light intensity of the light source varies according to a viscosity change of the fluid.

23. The thromboelastography instrument according to claim 22 , wherein the analyzing structure comprises a photo detector, wherein the photo detector detects the light intensity varying with the viscosity change of the fluid.

24. The thromboelastography instrument according to claim 13 , wherein the analyzing structure comprises a screen, wherein the screen demonstrates information and the screen allows user to give an input to change parameters.

25. The thromboelastography instrument according to claim 24 , wherein the screen is a touch screen.

26. The thromboelastography instrument according to claim 13 , wherein a maximum size of a plurality of micro-pillars is provided in a diameter range of 30 μm, a height of 80 μm and a center to center spacing of 55 μm between the plurality of micro-pillars in a micro-pillar matrix.

27. The thromboelastography instrument according to claim 13 , wherein the microfluidic channels are configured to provide different chemicals on different microfluidic channel paths to allow different tests being performed within a single cartridge.

28. The thromboelastography instrument according to claim 13 , wherein the cartridge is configured to provide regulation of a blood flow with a single micro-pillar, to affect mixing with at least one chemical and to make measurements on the single micro-pillar.

29. The thromboelastography instrument according to claim 13 , wherein at least one of a plurality of mixing protrusions is located on side walls of the cartridge.

30. The thromboelastography instrument according to claim 13 , wherein at least one of a plurality of micro-pillars is located on side walls of the cartridge.

31. The thromboelastography instrument according to claim 13 , wherein at least one of a plurality of micro-pillars is provided in a form of a rectangular prism.

32. The thromboelastography instrument according to claim 13 , wherein at least one of a plurality of micro-pillars is provided in a form of a cylinder.

Assignees

• Univ Koc

Inventors

• Yalcin Ozlem
• Erten Ahmet
• Tanyeri Melikhan

Classifications

• B01L3/50273 Primary

  characterised by the means or forces applied to move the fluids · CPC title

• G01N11/04

  through a restricted passage, e.g. tube, aperture · CPC title

• G01N33/4905 Primary

  Determining clotting time of blood (by chemical methods G01N33/86, C12Q1/56) · CPC title

• B01L2300/0832

  cylindrical, tube shaped · CPC title

• B01L2300/0877

  Flow chambers · CPC title