Coated flow path components for chromatographic effects

Patent metadata

Field	Value
Publication number	US-11994499-B2
Application number	US-202016800454-A
Country	US
Kind code	B2
Filing date	Feb 25, 2020
Priority date	Feb 27, 2019
Publication date	May 28, 2024
Grant date	May 28, 2024

Abstract

The present disclosure relates to the determination of analytes in a sample using chromatography. The present disclosure provides methods of separating an analyte from a sample. The method includes introducing a sample comprising the analytes into a chromatographic system. The chromatographic system has a flow path disposed in an interior of the chromatographic system, at least a portion of the flow path having an active coating, and a chromatographic column having a stationary phase material in an interior of the chromatographic column that facilitates separation of the analytes in the sample through interaction with at least one analyte in the sample. The active coating is selected to interact with at least one analyte in the sample through (1) a repulsive force, (2) a secondary interaction, or (3) a retention mechanism distinct from the interaction with the stationary phase material.

First claim

What is claimed is:

1. A method of separating analytes in a sample, the method comprising:
introducing a sample comprising the analytes into a chromatographic system comprising:
metallic flow path components disposed in an interior of the chromatographic system defining a flow path, at least a portion of the metallic flow path components having an active coating; and
a chromatographic column having a stationary phase material in an interior of the chromatographic column that facilitates separation of the analytes in the sample through interaction with at least one analyte in the sample, wherein the stationary phase material is distinct from the metallic flow path components;

wherein the metallic flow path components having the active coating are not inert to the analytes; and
wherein the active coating comprises an alkylsilyl coating of Formula I:

wherein each X is independently selected from (C 1 -C 6 )alkoxy, —NH(C 1 -C 6 )alkyl, —N((C 1 -C 6 )alkyl) 2 , OH, OR A , R B , R C , R D and halo;
R A represents a point of attachment to the interior surfaces of the fluidic system and at least one X is OR A ;
R B is absent or represents a hydrophobicity modifier;
R C represents a charge modifier and at least one X is R C ;
R D is absent, a chelator, or a crown ether; and
Y is a bridging moiety selected from (C 1 -C 20 )alkyl, —O[(CH 2 ) 2 O] 1-20 —, —(C 1 -C 10 )[NH(CO)NH(C 1 -C 10 )] 1-20 —, or —(C 1 -C 10 )[alkylphenyl(C 1 -C 10 )alkyl] 1-20 —.

2. The method of claim 1 , wherein the active coating is applied to the flow path by vapor deposition.

3. The method of claim 1 , wherein the active coating is applied to a frit.

4. The method of claim 3 , wherein the frit is positioned at an outlet of the chromatographic column.

5. The method of claim 3 , wherein the frit is positioned at an inlet of the chromatographic column.

Assignees

• Waters Technologies Corp

Inventors

• Lauber Matthew A
• Gilar Martin
• Delano Mathew H
• Donegan Michael

Classifications

• G01N30/26 Primary

  Conditioning of the fluid carrier; Flow patterns · CPC title

• B01D15/1878

  for multi-dimensional chromatography · CPC title

• B01D15/22

  relating to the construction of the column · CPC title

• B01D15/361

  Ion-exchange · CPC title

• B01D15/3847

  Multimodal interactions · CPC title