Metabolome sampling and analysis method for analyzing metabolome during synthetic gas fermentation of synthetic gas fermentation microorganisms
US-2020393427-A1 · Dec 17, 2020 · US
Metabolome sampling and analysis method for analyzing metabolome during synthetic gas fermentation of synthetic gas fermentation microorganisms
US11988651B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11988651-B2 |
| Application number | US-201916975581-A |
| Country | US |
| Kind code | B2 |
| Filing date | Feb 21, 2019 |
| Priority date | Feb 28, 2018 |
| Publication date | May 21, 2024 |
| Grant date | May 21, 2024 |
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Abstract
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The present invention relates to a metabolome sampling and analysis method for analyzing metabolome during synthetic gas fermentation of a synthetic gas fermentation microorganisms, the method establishing an optimal condition for metabolome sampling and enabling a glucose culture and a synthetic gas culture of the synthetic gas fermentation microorganisms to be distinguished by using a selected metabolomic biomarker.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method for analyzing metabolomic differentiation for distinguishing a glucose culture and a synthetic gas culture of Clostridium carboxidivorans , the method comprising: a metabolome sampling step of subjecting a biological sample of Clostridium carboxidivorans to fast filtration under an aerobic condition, washing the filtrate with water, and then extracting a metabolome using a mixed solvent of water, 2-propanol, and methanol at a volume ratio of 2:2:5 as an extraction solvent.
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Classifications
Metabolic gas from microbes, cell cultures or plant tissues · CPC title
- G01N30/7206Primary
interfaced to gas chromatograph (interfaces in general for introducing or extracting samples to be analysed with specially adapted mass spectrometer, see H01J49/04) · CPC title
in the gaseous state {(specially adapted for biological material G01N33/497; measuring breath flow A61B5/087)} · CPC title
Preparation · CPC title
Evaluation, i.e. decoding of the signal into analytical information (for analysis of specific compounds see also G01N30/88 and subgroups of G01N33/00; chemical libraries per se C40B) · CPC title
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- What does patent US11988651B2 cover?
- The present invention relates to a metabolome sampling and analysis method for analyzing metabolome during synthetic gas fermentation of a synthetic gas fermentation microorganisms, the method establishing an optimal condition for metabolome sampling and enabling a glucose culture and a synthetic gas culture of the synthetic gas fermentation microorganisms to be distinguished by using a selecte…
- Who is the assignee on this patent?
- Univ Korea Res & Bus Found, Korea Inst Sci & Tech
- What technology area does this patent fall under?
- Primary CPC classification G01N30/7206. Mapped technology areas include Physics.
- When was this patent published?
- Publication date Tue May 21 2024 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 4 related publications on this page (citations in our corpus or others sharing the same primary CPC).