Sampling system and method of using the same

Patent metadata

Field	Value
Publication number	US-11987784-B2
Application number	US-202117348961-A
Country	US
Kind code	B2
Filing date	Jun 16, 2021
Priority date	Jun 16, 2020
Publication date	May 21, 2024
Grant date	May 21, 2024

Abstract

The present application is directed to a sampling system for sampling a fluid from a vessel, where the sampling system includes a sterile dispenser assembly operatively connected to the vessel, the sterile dispenser assembly including a valve operatively connected to the vessel, a membrane, and a needle, and a detachable sterile sampling container assembly operatively connected to the sterile dispenser assembly, the detachable sterile sampling container assembly including a sampling container, a membrane attached to the sampling container, and a sampling container housing enclosing the sampling container, where the sampling container housing includes a compressible portion having a deflated configuration and an expanded configuration.

First claim

What is claimed:

1. A sampling system for sampling a fluid from a vessel, wherein the sampling system comprises:
a sterile dispenser assembly operatively connected to the vessel, the sterile dispenser assembly comprising a valve operatively connected to the vessel, a membrane, and a needle, and
a detachable sterile sampling container assembly operatively connected to the sterile dispenser assembly, the detachable sterile sampling container assembly comprising a sampling container, a membrane attached to the sampling container, and a sampling container housing enclosing the sampling container,
wherein the sampling container housing comprises a compressible portion having a deflated configuration and an expanded configuration, and
wherein, when the compressible portion is in a deflated configuration, the compressible portion pushes the sampling container of the sterile sampling container assembly through the membrane of the sterile sampling container assembly and into proximity with the needle, allowing the needle to dispense the fluid into the sampling container while maintaining a closed aseptic system.

2. The sampling system of claim 1 , wherein the fluid comprises a biological media comprising cells.

3. The sampling system of claim 1 , wherein the vessel comprises a cell culture container.

4. The sampling system of claim 1 , wherein the membrane of the sterile sampling container assembly comprises a breathable valve membrane.

5. The sampling system of claim 1 , wherein the membrane of the sterile dispenser assembly comprises a breathable valve membrane.

6. The sampling system of claim 5 , wherein the breathable valve membrane comprises a silicone.

7. The sampling system of claim 1 , wherein the sampling system comprises a plurality of sterile dispenser assemblies operatively connected to the vessel.

8. The sampling system of claim 1 , wherein the sampling system is capable of undergoing autoclave sterilization, ethylene oxide sterilization, or gamma sterilization.

9. The sampling system of claim 1 , wherein the container housing comprises a transparent or translucent material.

10. The sampling system of claim 1 , further comprising a disposable cap overlying at least one of the membrane or sampling container housing.

11. The sampling system of claim 1 , wherein the sampling container housing comprises a top portion disposed above the compressible portion and a bottom portion disposed below the compressible portion.

12. The sampling system of claim 11 , wherein at least one of the top portion or the bottom portion is detachable from the compressible portion.

13. The sampling system of claim 1 , further comprising a disposable cap overlying at least one of the membrane or valve of the sterile dispenser assembly.

14. The sampling system of claim 1 , wherein the needle comprises a needle sleeve.

15. The sampling system of claim 1 , wherein the compressible portion comprises an elastomer.

16. The sampling system of claim 1 , wherein the sampling container comprises a sampling container cap.

17. A sampling system for sampling a fluid from a vessel, wherein the sampling system comprises:
a sterile dispenser assembly operatively connected to the vessel, the sterile dispenser assembly comprising a valve, a membrane, and a needle, wherein the valve comprises a valve body and a valve stem disposed at least partially within the valve body, the valve stem comprising a sidewall defining a central lumen and at least one opening in the sidewall, wherein the valve is adapted to prevent fluid flow through the lumen when the at least one opening is disposed within the valve body and permit fluid flow through the lumen when the at least one opening is exposed from the valve body, wherein the valve is essentially free of a spring, and wherein the needle is operatively connected to the valve to dispense fluid from the vessel.

18. A sampling system for sampling a fluid from a vessel, wherein the sampling system comprises:
a detachable sterile sampling container assembly comprising a sampling container, a membrane attached to the sampling container, and a sampling container housing enclosing the sampling container,
wherein the sampling container housing comprises a compressible portion having a deflated configuration and an expanded configuration, and
wherein, when the compressible portion is in a deflated configuration, the compressible portion pushes the sampling container through the membrane into proximity with a needle, allowing the needle to dispense the fluid into the sampling container while maintaining a closed aseptic system.

19. The sampling system of claim 18 , wherein the sterile sampling container assembly further comprises a needle operatively connected to the membrane or sampling container housing.

Assignees

• Saint Gobain Performance Plastics Corp

Inventors

• Zoellner Clemens E
• Nixon Thomas R
• Wheatley Jonathon

Classifications

• C12M33/04 Primary

  by injection or suction, e.g. using pipettes, syringes, needles (pipettes in general B01L3/02) · CPC title

• B01L3/0293

  for liquids · CPC title

• B01L3/502

  with fluid transport, e.g. in multi-compartment structures · CPC title

• B01L13/00

  Cleaning or rinsing apparatus · CPC title

• C12M23/22

  Transparent or translucent parts (glassware for laboratory use B01L3/00) · CPC title