Biochemical carriers capable of storage, preservation and indexing and method for fabricating the same

Patent metadata

Field	Value
Publication number	US-11987693-B2
Application number	US-201716335296-A
Country	US
Kind code	B2
Filing date	Oct 10, 2017
Priority date	Oct 5, 2016
Publication date	May 21, 2024
Grant date	May 21, 2024

Abstract

Biochemical carriers are provided. Each of the biochemical carriers includes: biochemical molecules having a sequence into which digital data information is encoded; a carrier particle composed of a polymer matrix and in which the biochemical molecules are connected to the surface or inside of the polymer matrix; and an index code introduced into the carrier particle. Also provided is a method for fabricating biochemical carriers. The fabrication method includes: encoding digital data into a sequence of biochemical molecules; synthesizing the biochemical molecules based on the encoded sequence; mixing the biochemical molecules with a photocurable material; curing the mixture to obtain carrier particles including a polymer matrix; and introducing an index code into the carrier particles simultaneously with or separately from the curing. Also provided is a method for restoring digital data from the biochemical carrier. The restoration method includes: analyzing the index code of the biochemical carrier; reacquiring the biochemical molecules from the biochemical carrier based on the analytical results of the index code; sequencing the biochemical molecules; and decoding the sequencing results to restore digital data.

First claim

What is claimed is:

1. A biochemical carrier comprising: biochemical molecules having a sequence into which digital data information is encoded; a carrier particle composed of a polymer matrix and in which the biochemical molecules are connected to the surface or inside of the polymer matrix; and an index code introduced into the carrier particle.

2. The biochemical carrier according to claim 1 , wherein the polymer matrix is composed of a photocurable polymer.

3. The biochemical carrier according to claim 1 , wherein the polymer matrix is porous.

4. The biochemical carrier according to claim 1 , wherein the biochemical molecules have functional groups that are chemically connected to the chains of the polymer matrix.

5. The biochemical carrier according to claim 1 , further comprising a protective layer surrounding the surface of the carrier particle.

6. The biochemical carrier according to claim 5 , wherein the protective layer is formed using a metal or metal oxide.

7. The biochemical carrier according to claim 5 , wherein the protective layer is a silica shell that is connected to the surface of the carrier particle via —Si—O—Si— bonds.

8. The biochemical carrier according to claim 1 , wherein the carrier particle has a volume of 10 −9 to 1 mm 3 .

9. The biochemical carrier according to claim 1 , wherein the index code contains information about the biochemical molecules connected to the carrier particle, a method for reacquiring and analyzing the biochemical molecules to decode encoded digital information or a method for accessing to the decoding method.

10. The biochemical carrier according to claim 1 , wherein the index code is selected from the group consisting of QR codes, binary codes, graphical codes, spectral codes, and topographical codes.

11. A method for fabricating biochemical carriers, comprising: encoding digital data into a sequence of biochemical molecules; synthesizing the biochemical molecules based on the encoded sequence; mixing the biochemical molecules with a photocurable material; curing the mixture to obtain carrier particles comprising a polymer matrix; and introducing an index code into the carrier particles simultaneously with or separately from the curing.

12. The method according to claim 11 , further comprising forming protective layers surrounding the carrier particles.

13. The method according to claim 11 , wherein the biochemical molecules have functional groups that are chemically connected to the chains of the polymer matrix.

14. The method according to claim 11 , wherein the mixture further comprises a porogen.

15. The method according to claim 14 , wherein the porogen is a polyalkylene glycol.

16. The method according to claim 11 , wherein the carrier particles are obtained by optofluidic maskless lithography.

17. A method for restoring digital data from a biochemical carrier comprising:
analyzing the index code of the biochemical carrier;
wherein
the biochemical carrier comprises:
biochemical molecules having a sequence into which digital data information is encoded;
a carrier particle composed of a polymer matrix and in which the biochemical molecules are connected to the surface or inside of the polymer matrix; and
an index code introduced into the carrier particle,
reacquiring the biochemical molecules from the biochemical carrier based on the analytical results of the index code;
sequencing the biochemical molecules; and
decoding the sequencing results to restore digital data.

18. The method according to claim 17 , wherein the carrier particles stores a plurality of data sets and a desired data set is selectively restored from the plurality of data sets.

Assignees

• Seoul Nat Univ R&Db Foundation
• Univ Industry Cooperation Group Kyung Hee Univ

Inventors

• Kwon Sung Hoon
• Park Wook
• Choi Yeong Jae
• Bae Hyung Jong
• Ryu Tae Hoon
• Song Suk Heung

Classifications

• C08L35/02 Primary

  Homopolymers or copolymers of esters (C08L35/06, C08L35/08 take precedence) · CPC title

• C08J3/128

  Polymer particles coated by inorganic and non-macromolecular organic compounds · CPC title

• C08J9/286

  the liquid phase being a solvent for the monomers but not for the resulting macromolecular composition, i.e. macroporous or macroreticular polymers · CPC title

• C12N15/11

  DNA or RNA fragments; Modified forms thereof (DNA or RNA not used in recombinant technology, C07H21/00); {Non-coding nucleic acids having a biological activity} · CPC title

• G06F16/2237

  Vectors, bitmaps or matrices · CPC title