Camelidae single-domain antibodies against Yersinia pestis and methods of use

Patent metadata

Field	Value
Publication number	US-11987618-B2
Application number	US-202117141477-A
Country	US
Kind code	B2
Filing date	Jan 5, 2021
Priority date	May 31, 2012
Publication date	May 21, 2024
Grant date	May 21, 2024

Abstract

Single-domain antibodies (SAbs) against three Yersinia pestis surface proteins (LcrV, YscF, and F1), nucleic acid sequences encoding the SAbs, and polypeptides comprising two or more SAbs capable of recognizing two or more epitopes and/or antigens. The present invention further includes methods for preventing or treating Y. pestis infections in a patient; methods for detecting and/or diagnosing Y. pestis infections; and devices and methods for identifying and/or detecting Y. pestis on a surface and/or in an environment.

First claim

What is claimed is:

1. A single-domain antibody against Yersinia pestis ( Y. pestis ) LcrV protein comprising:
a first framing region (“FR”) sequence comprising SEQ ID No:97 or SEQ ID No:98;
a first complementarity determining region (“CDR”) sequence comprising SEQ ID No:22;
a second FR sequence comprising SEQ ID No:116, the first CDR sequence being positioned between the first FR sequence and the second FR sequence;
a second CDR sequence comprising SEQ ID No:49;
a third FR sequence comprising SEQ ID No: 141, the second CDR sequence being positioned between the second FR sequence and the third FR sequence;
a third CDR sequence comprising SEQ ID No.73; and
a fourth FR sequence comprising SEQ ID No:148, the third CDR sequence being positioned between the third FR sequence and the fourth FR sequence.

2. The single-domain antibody of claim 1 , further comprising:
at least one of a protein tag, a protein domain tag, or a chemical tag.

Assignees

• Us Gov Air Force
• The Government Of The Us Secretary Of The Air Force

Inventors

• Mauzy Camilla A
• Muyldermans Serge Victor Marie

Classifications

• C07K16/1228 Primary

  Enterobacterales (O), e.g. Citrobacter (G), Serratia (G), Proteus (G), Providencia (G), Morganella (G) or Yersinia (G) · CPC title

• C07K2317/22

  from camelids, e.g. camel, llama or dromedary · CPC title

• C07K2317/569

  Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody® · CPC title

• C07K2317/92

  Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value · CPC title