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Polypeptide and method of producing IMP using the same
US11987611B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11987611-B2 |
| Application number | US-202318154296-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jan 13, 2023 |
| Priority date | Dec 15, 2017 |
| Publication date | May 21, 2024 |
| Grant date | May 21, 2024 |
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- Title
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Abstract
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The present disclosure relates to a novel polypeptide having an activity of exporting 5′-inosine monophosphate, a microorganism comprising the same, a method for preparing 5′-inosine monophosphate using the same, and a method for increasing export of 5′-inosine monophosphate.
First claim
Opening claim text (preview).
The invention claimed is: 1. A microorganism of the genus Corynebacterium producing 5′-inosine monophosphate, which comprises a protein variant exporting 5′-inosine monophosphate; the polynucleotide encoding the protein variant; or a vector comprising the polynucleotide encoding the protein variant; wherein, in the amino acid sequence of SEQ ID NO: 2, (i) the 2 nd amino acid, (ii) the 64 th amino acid, or (iii) the 2 nd amino acid and the 64 th amino acid are each substituted with another amino acid. 2. The microorganism of the genus Corynebacterium according to claim 1 , wherein the 2 nd amino acid is substituted with an amino acid selected from the group consisting of isoleucine, phenylalanine, methionine, glutamic acid, histidine, and asparagine; (ii) the 64 th amino acid is substituted with an amino acid selected from the group consisting of aspartate, glutamic acid, asparagine, cysteine, isoleucine, and phenylalanine; or (iii) the 2 nd amino acid and the 64 th amino acid are each substituted with an amino acid selected from the group consisting of methionine, glutamic acid, histidine, asparagine, aspartate, cysteine, isoleucine, and phenylalanine. 3. The microorganism of the genus Corynebacterium according to claim 1 , wherein the microorganism of the genus Corynebacterium is Corynebacterium stationis. 4. A method for preparing 5′-inosine monophosphate, comprising culturing the microorganism of the genus Corynebacterium of claim 1 in a medium; and recovering 5′-inosine monophosphate from the microorganism or medium. 5. The method according to claim 4 , wherein the microorganism of the genus Corynebacterium is Corynebacterium stationis. 6. The microorganism of the genus Corynebacterium according to claim 1 , further comprising a protein variant in which the 164th amino acid of SEQ ID NO: 1 is substituted with another amino acid; the polynucleotide encoding the protein variant; or a vector comprising the polynucleotide encoding the protein variant. 7. The microorganism of the genus Corynebacterium according to claim 6 , wherein the 164 th amino acid of SEQ ID NO: 1 is substituted with an amino acid selected from the group consisting of lysine, arginine, asparagine, glycine, threonine, and proline. 8. The microorganism of the genus Corynebacterium according to claim 6 , wherein the microorganism of the genus Corynebacterium is Corynebacterium stationis. 9. A method for preparing 5′-inosine monophosphate, comprising culturing the microorganism of the genus Corynebacterium of claim 6 in a medium; and recovering 5′-inosine monophosphate from the microorganism or medium. 10. The method according to claim 9 , wherein the microorganism of the genus Corynebacterium is Corynebacterium stationis.
Assignees
Inventors
Classifications
- C07K14/705Primary
Receptors; Cell surface antigens; Cell surface determinants {(tumour specific antigens C07K14/4748)} · CPC title
from Corynebacterium (G) · CPC title
Bacterial isolates · CPC title
DNA or RNA fragments; Modified forms thereof (DNA or RNA not used in recombinant technology, C07H21/00); {Non-coding nucleic acids having a biological activity} · CPC title
for Corynebacterium; for Brevibacterium · CPC title
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Frequently asked questions
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- What does patent US11987611B2 cover?
- The present disclosure relates to a novel polypeptide having an activity of exporting 5′-inosine monophosphate, a microorganism comprising the same, a method for preparing 5′-inosine monophosphate using the same, and a method for increasing export of 5′-inosine monophosphate.
- Who is the assignee on this patent?
- Cj Cheiljedang Corp
- What technology area does this patent fall under?
- Primary CPC classification C07K14/705. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue May 21 2024 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 5 related publications on this page (citations in our corpus or others sharing the same primary CPC).