System and method for sperm sorting

Patent metadata

Field	Value
Publication number	US-11982610-B2
Application number	US-202318371952-A
Country	US
Kind code	B2
Filing date	Sep 22, 2023
Priority date	Nov 20, 2013
Publication date	May 14, 2024
Grant date	May 14, 2024

Abstract

A system and method for sorting sperm is provided. The system includes a housing and a microfluidic system supported by the housing. The system also includes an inlet providing access to the microfluidic system to deliver sperm to the microfluidic system and an outlet providing access to the microfluidic system to harvest sorted sperm from the microfluidic system. The microfluidic system provides a flow path for sperm from the inlet to the outlet and includes at least one channel extending from the inlet to the outlet to allow sperm delivered to the microfluidic system through the inlet to progress along the flow path toward the outlet. The microfluidic system also includes a filter including a first plurality of micropores arranged in the flow path between the inlet and the outlet to cause sperm traveling along the flow path to move against through the filter and gravity to reach the outlet.

First claim

The invention claimed is:

1. A system for sorting sperm, comprising:
a chip comprising a lower component layer and an upper component layer affixed together with an adhesive;
a fluidic system within the chip;
an inlet extending through the lower component layer and providing access to the fluidic system to deliver sperm to the fluidic system;
a filter chamber configured to pass motile sperm for harvesting and restrict non-motile sperm, the filter chamber comprising a first chamber and a second chamber positioned above the first chamber;
at least one straight channel directly connecting the inlet to the first chamber to allow sperm delivered to the fluidic system through the inlet to progress along a flow path toward the filter chamber;
a filter comprising a plurality of micropores sized to permit a head of the sperm to pass therethrough, and arranged in the filter chamber to cause sperm traveling along the flow path to move through the filter and against gravity to reach the second chamber, wherein the filter is located between the upper component layer and the lower component layer; and
an additional collection chamber extending into the upper component layer and connected to the second chamber by an open channel, the additional collection chamber sized and configured to facilitate harvesting functional sperm out of the additional collection chamber.

2. The system of claim 1 , further comprising a substrate layer located under the lower component layer.

3. The system of claim 1 , further comprising a slide located under the lower component layer.

4. The system of claim 3 , wherein the slide is a glass slide.

5. The system of claim 1 , wherein the first chamber extends through the lower component layer.

6. The system of claim 5 , wherein the second chamber extends through the upper component layer.

7. The system of claim 1 , wherein the plurality of micropores are at least 1 lam and less than 10 μm in diameter.

8. The system of claim 1 , wherein the plurality of micropores are greater than 4.5 μm in diameter.

9. The system of claim 1 , wherein the lower component layer has a flat upper surface and a flat lower surface.

10. The system of claim 1 , wherein the lower component layer has a flat lower component upper surface and a flat lower component lower surface and wherein the upper component layer has a flat upper component upper surface and a flat upper component lower surface.

11. The system of claim 1 , wherein the inlet extends through both the lower component layer and the upper component layer.

12. The system of claim 1 , wherein the additional collection chamber does not comprise an additional filter connected to an outlet.

13. The system of claim 1 , wherein the filter is a polycarbonate filter, and wherein the second chamber is connected to the additional collection chamber by a fluid connection channel and no other channels extend from the second chamber.

14. A method for sorting sperm, comprising:
delivering a sample of sperm into an inlet connected to a fluidic system within a chip comprising a lower component layer and an upper component layer affixed together with an adhesive, wherein the inlet extends through the lower component layer and provides access to the fluidic system to deliver sperm to the fluidic system;
allowing at least some motile sperm in the sample of sperm to traverse a flow path through the fluidic system from the inlet to a filter chamber to form a population of motile sperm that have entered the filter chamber,
wherein the filter chamber comprises a first chamber and a second chamber positioned above the first chamber;
wherein at least one channel connects the inlet to the first chamber,
wherein the first chamber and the second chamber are separated by a filter positioned in the filter chamber,
wherein the filter comprises a plurality of micropores sized to permit a head of the sperm to pass therethrough, and
wherein the second chamber is connected to an additional collection chamber by a fluid connection channel;
allowing at least some motile sperm from the population of motile sperm that have entered the filter chamber to selectively pass through the filter against gravity to traverse a path into the second chamber and then to traverse a path into the additional collection chamber in a flow-free manner; and
harvesting functional sperm from the additional collection chamber.

15. The method of claim 14 , wherein the first chamber extends through the lower component layer.

16. The method of claim 15 , wherein the second chamber extends through the upper component layer.

17. The method of claim 14 , wherein the inlet extends through both the lower component layer and the upper component layer.

18. The method of claim 14 , wherein the filter is located between the upper component layer and the lower component layer.

19. The method of claim 14 , wherein the filter chamber is connected to the additional collection chamber by an open fluid connection when the sample of sperm is delivered into the inlet, and wherein some of the sperm in the sample of sperm traverse a path through the open fluid connection into the additional collection chamber in a flow-free manner.

20. The method of claim 14 , wherein a higher percentage of motile sperm are present at the additional collection chamber than in the sample of sperm when functional sperm are harvested.

21. The method of claim 14 , wherein the plurality of micropores are at least 1 μm and less than 10 μm in diameter.

22. The method of claim 14 , wherein the at least one channel that directly connects the inlet to the first chamber is a straight channel that directly connects the inlet to the first chamber, and wherein the plurality of micropores are greater than 4.5 μm.

23. The method of claim 14 , wherein the plurality of micropores are 5 μm to 8 μm in diameter.

24. The method of claim 23 , wherein the sperm are human sperm.

25. The method of claim 14 , wherein the lower component layer has a flat lower component upper surface and a flat lower component lower surface, and wherein the method further comprises using the harvested sperm in an intrauterine insemination (IUI) procedure.

26. The method of claim 25 , wherein the filter is located between the upper component layer and the lower component layer.

27. The method of claim 26 , wherein the sperm are human sperm, wherein the plurality of micropores are at least 4.5 μm in diameter, and wherein a higher percentage of motile sperm are present at the additional collection chamber than in the sample of sperm when functional sperm are harvested.

28. The method of claim 27 , wherein the inlet extends through both the lower component layer and the upper component layer.

29. A method for sorting sperm, comprising:
delivering a sample of sperm into an inlet connected to a fluidic system within a chip comprising a lower component layer and an upper component layer affixed together with an adhesive, wherein the lower component layer has a flat upper surface and a flat lower surface, and wherein the inlet provides access to the fluidic system to deliver sperm to the fluidic system;
allowing at least some motile sperm in the sample of sperm to traverse a flow path through the fluidic system from the inlet to a filter chamber and towards an outlet to form a population of motile sperm that have entered the filte

Assignees

• Brigham & Womens Hospital Inc

Inventors

• Demirci Utkan
• Asghar Waseem

Classifications

• G01N15/10 Primary

  Investigating individual particles · CPC title

• B01L3/502753

  characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation · CPC title

• C12N5/061

  Sperm cells, spermatogonia · CPC title

• C12N5/0612

  sorting of gametes, e.g. according to sex or motility · CPC title

• G01N1/4005

  by transferring a selected component through a membrane · CPC title