Methods for generation of podocytes from pluripotent stem cells and cells produced by the same

Patent metadata

Field	Value
Publication number	US-11980641-B2
Application number	US-202117366827-A
Country	US
Kind code	B2
Filing date	Jul 2, 2021
Priority date	Nov 25, 2014
Publication date	May 14, 2024
Grant date	May 14, 2024

Abstract

Embodiments of various aspects described herein relate to methods, kits, and cell culture media for generation of podocytes from pluripotent stem (PS) cells, as well as cells produced by the same, and methods of use.

First claim

The invention claimed is:

1. A method of screening an agent that reduces at least one phenotypic characteristic of podocytes associated with at least one of kidney disorder and glomerular disorder, comprising:
a) providing an agent and podocytes, wherein said podocytes are in a microfluidic device and have phenotypic characteristics of being substantially negative for Paired Box 2 (PAX-2) protein, exhibiting an increased uptake of exogenous albumin compared to immortalized podocytes, and exhibiting extension of podocyte foot processes;
b) contacting said podocytes with said agent to produce contacted podocytes; and
c) detecting a reduction in at least one of said phenotypic characteristics of said contacted podocytes produced by step b) compared to said podocytes of step a), thereby identifying said agent as reducing at least one phenotypic characteristic of podocytes associated with at least one of kidney disorder and glomerular disorder.

2. The method of claim 1 , wherein said agent comprises a protein.

3. The method of claim 1 , wherein said podocytes are derived from intermediate mesoderm cells.

4. The method of claim 1 , wherein said agent comprises a peptide.

5. The method of claim 1 , wherein said agent comprises a nucleic acid.

6. The method of claim 1 , wherein said agent comprises a small molecule.

7. The method of claim 1 , wherein said agent comprises a drug.

Assignees

• Harvard College

Inventors

• Ingber Donald E
• Musah Samira

Classifications

• A61K35/22 Primary

  Urine; Urinary tract, e.g. kidney or bladder; Intraglomerular mesangial cells; Renal mesenchymal cells; Adrenal gland · CPC title

• C12N5/0686 Primary

  Kidney cells · CPC title

• A61K35/545

  Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Uncharacterised stem cells · CPC title

• C12N2501/155

  Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor · CPC title

• C12N2501/16

  Activin; Inhibin; Mullerian inhibiting substance · CPC title