Flow cells

Patent metadata

Field	Value
Publication number	US-11975478-B2
Application number	US-202117235244-A
Country	US
Kind code	B2
Filing date	Apr 20, 2021
Priority date	Apr 24, 2020
Publication date	May 7, 2024
Grant date	May 7, 2024

Abstract

An example of a flow cell includes a substrate and a cured, patterned resin on the substrate. The cured, patterned resin has nano-depressions separated by interstitial regions. Each nano-depression has a largest opening dimension ranging from about 10 nm to about 1000 nm. The cured, patterned resin also includes an interpenetrating polymer network. The interpenetrating polymer network of the cured, patterned resin includes an epoxy-based polymer and a (meth)acryloyl-based polymer.

First claim

What is claimed is:

1. A flow cell, comprising:
a substrate; and
a cured, patterned resin on the substrate, the cured, patterned resin including nano-depressions separated by interstitial regions, the nano-depressions each having a largest opening dimension ranging from about 10 nm to about 1000 nm, and the cured, patterned resin including an interpenetrating polymer network including an epoxy-based polymer and a (meth)acryloyl-based polymer, wherein a weight ratio of the epoxy-based polymer to the (meth)acryloyl-based polymer ranges from about 25:75 to about 75:25.

2. The flow cell as defined in claim 1 , further comprising:
a grating layer positioned on the substrate; and
a planar waveguide layer positioned on the grating layer.

3. The flow cell as defined in claim 2 , wherein:
a refractive index of the interpenetrating polymer network ranges from about 1.35 to about 1.52; and
a refractive index of the planar waveguide layer ranges from about 1.6 to about 2.5.

4. The flow cell as defined in claim 1 , further comprising:
a hydrogel positioned in each of the nano-depressions; and
amplification primers attached to the hydrogel.

5. The flow cell as defined in claim 1 , wherein a thickness of the cured, patterned resin ranges from about 225 nm to about 600 nm.

6. The flow cell as defined in claim 1 , wherein:
the epoxy-based polymer is formed from a multi-functional epoxy monomer and the multi-functional epoxy monomer is Poly(propylene glycol) diglycidyl ether:

the (meth)acryloyl-based polymer is formed from a multi-functional (meth)acryloyl monomer and the multi-functional (meth)acryloyl monomer is 2,2,3,3,4,4,5,5-octafluoro-1,6-hexanediol diacrylate:

and
the predetermined weight ratio is about 50:50.

7. The flow cell as defined in claim 1 , wherein:
the epoxy-based polymer is formed from a multi-functional epoxy monomer and the multi-functional epoxy monomer is Poly(propylene glycol) diglycidyl ether:

the (meth)acryloyl-based polymer is formed from a multi-functional (meth)acryloyl monomer and the multi-functional (meth)acryloyl monomer is Glycerol 1,3-diglycerolate diacrylate:

and
the predetermined weight ratio ranges from about 25:75 to about 75:25.

8. A method, comprising:
applying a resin mixture on a substrate of a flow cell, the resin mixture including a multi-functional epoxy monomer and a multi-functional (meth)acryloyl monomer at a weight ratio ranging from about 25:75 to about 75:25;
imprinting the resin mixture with a working stamp having a plurality of nano-features; and
curing the resin mixture while the working stamp is in place, thereby forming an interpenetrating polymer network including an epoxy-based polymer and a (meth)acryloyl-based polymer at a weight ratio ranging from about 25:75 to about 75:25 that is imprinted with flow cell nano-depressions separated by interstitial regions, the nano-depressions each having a largest opening dimension ranging from about 10 nm to about 1000 nm.

9. The method as defined in claim 8 , wherein the multi-functional epoxy monomer is selected from the group consisting of:
i) 2,4,6,8-tetramethyl-2,4,6,8-tetrakis(propyl glycidyl ether)cyclotetrasiloxane:

ii) Tetrakis(epoxycyclohexyl ethyl)tetramethyl cyclotetrasiloxane:

iii) Poly(dimethylsiloxane), diglycidyl ether terminated:

wherein 4<n<8;
iv) Poly(propylene glycol) diglycidyl ether:

wherein 5<n<10;
v) 3,4-Epoxycyclohexylmethyl 3,4-epoxycyclohexanecarboxylate:

vi) Bisphenol A diglycidyl ether, brominated:

vii) Poly(Bisphenol A-co-epichlorohydrin), glycidyl end-capped:

wherein 0<n<2;
viii) Bisphenol A propoxylate diglycidyl ether:

ix) Monophenyl functional tris(epoxy terminated polydimethylsiloxane):

x) Trimethylolpropane triglycidyl ether:

xi) 2,2′-(2,2,3,3,4,4,5,5-Octafluorohexane-1,6-diyl)bis(oxirane):

xii) 1,3-Bis(3-glycidoxypropyl)tetramethyldisiloxane:

xiii) 1,3 Bis[2(3,4 epoxycyclohex-1-yl)ethyl]tetra-methyldisiloxane:

xiv) Glycidyl polyoctahedral silsesquioxane:

and
xv) Epoxycyclohexyl polyoctahedral silsesquioxane:

xvi) Tris(4-hydroxyphenyl)methane triglycidyl ether:

xvii) 4,4′-Methylenebis(N,N-diglycidylaniline):

and
xviii) any combination of i) through xvii).

10. The method as defined in claim 8 , wherein the multi-functional (meth)acryloyl monomer is selected from the group consisting of:
i) 2,2,3,3,4,4,5,5-octafluoro-1,6-hexanediol diacrylate:

ii) Pentaerythritol tetraacrylate:

iii) Pentaerythritol triacrylate:

iv) Glycerol 1,3-diglycerolate diacrylate:

v) Poly(ethylene glycol) dimethacrylate:

wherein 8<n<10;
vi) Glycerol dimethacrylate, mixture of isomers:

vii) 3-(Acryloyloxy)-2-hydroxypropyl methacrylate:

Assignees

• Illumina Cambridge Ltd

Inventors

• Richez Alexandre

Classifications

• B29C59/022 Primary

  characterised by the disposition or the configuration, e.g. dimensions, of the embossments or the shaping tools therefor · CPC title

• B01L3/5027 Primary

  by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip · CPC title

• B29C59/005

  characterised by the choice of material · CPC title

• B29C59/026

  of layered or coated substantially flat surfaces · CPC title

• C08G59/063

  with epihalohydrins · CPC title