Phage-derived particles for in situ delivery of DNA payload into C. acnes population

Patent metadata

Field	Value
Publication number	US-11970701-B2
Application number	US-202318477069-A
Country	US
Kind code	B2
Filing date	Sep 28, 2023
Priority date	Nov 4, 2020
Publication date	Apr 30, 2024
Grant date	Apr 30, 2024

Abstract

The invention relates to C. acnes carrying DNA vectors with a C. acnes phage packaging signal and a gene of interest. The invention encompasses a C. acnes producer cell carrying DNA vectors, with a C. acnes phage packaging signal and a gene of interest, for the production of phage-derived particles that can robustly transduce C. acnes receiver cell allowing transgene expression. The invention encompasses C. acnes phage-derived particles carrying these vectors, C. acnes containing these vectors or modified by transduction of these phage-derived particles, and methods of using these phage-derived particles.

First claim

We claim:

1. A C. acnes phage-derived particle comprising a phagemid lacking genes encoding phage structural proteins, said phagemid comprising:
a phage packaging signal allowing packaging of the phagemid in a Cutibacterium acnes phage capsid,
wherein the phage packaging signal is at least 87% identical to SEQ ID NO: 76, and
a gene of interest that is a transgene that is exogenous to C. acnes.

2. The C. acnes phage-derived particle of claim 1 , wherein the phagemid further comprises an origin of replication for C. acnes and a selection marker for C. acnes.

3. The C. acnes phage-derived particle of claim 1 , wherein the transgene encodes a CRISPR-Cas system.

4. The C. acnes phage-derived particle of claim 3 , wherein the CRISPR-Cas system targets a C. acnes chromosome locus which is a proinflammatory sequence related to acne vulgaris.

5. The C. acnes phage-derived particle of claim 1 , wherein said transgene encodes an interleukin.

6. The C. acnes phage-derived particle of claim 2 , wherein the selection marker is not ermE.

7. The C. acnes phage-derived particle of claim 2 , wherein the selection marker is catA.

8. The C. acnes phage-derived particle of claim 1 , wherein the phagemid comprises a DNA encoding an antigen.

Assignees

• Eligo Bioscience

Inventors

• Leveau Aymeric
• Canadas Blasco Inès
• Mathieu Aurélie
• Decrulle Antoine

Classifications

• C12N15/76 Primary

  for Actinomyces; for Streptomyces · CPC title

• A61K39/02

  Bacterial antigens · CPC title

• A61K39/05

  Actinobacteria, e.g. Actinomyces, Streptomyces, Nocardia, Bifidobacterium, Gardnerella}, Corynebacterium; Propionibacterium {(Mycobacterium A61K39/04) · CPC title

• A61P17/10

  Anti-acne agents · CPC title

• C12N9/22

  Ribonucleases {[RNase]; Deoxyribonucleases [DNase]} · CPC title