Apparatus for sensor-based examination of biological cells

Patent metadata

Field	Value
Publication number	US-11969730-B2
Application number	US-201816755114-A
Country	US
Kind code	B2
Filing date	Sep 28, 2018
Priority date	Oct 12, 2017
Publication date	Apr 30, 2024
Grant date	Apr 30, 2024

Abstract

Disclosed is an apparatus for the sensor-based examination of biological cells, comprising at least one well, wherein the at least one well has (a) an opening; (b) a planar base; (c) at least one sensor for the examination of biological cells, wherein the at least one sensor is arranged on the base of the at least one well; and (d) a medium for cryopreservation, with the medium for cryopreservation comprising biological cells and contacting a surface of the at least one sensor, wherein the medium for cryopreservation, which comprises the biological cells, is present in a frozen state.

First claim

The invention claimed is:

1. An apparatus for the sensor-based examination of biological cells, comprising at least one well, wherein the at least one well has
(a) an opening;
(b) a planar base;
(c) at least one sensor for the examination of biological cells, wherein the at least one sensor is arranged on the base of the at least one well; and
(d) a medium for cryopreservation, with the medium for cryopreservation comprising biological cells and contacting a surface of the at least one sensor, wherein the medium for cryopreservation, which comprises the biological cells, is present in a frozen state.

2. The apparatus of claim 1 , wherein the at least one sensor
(i) is selected from the group consisting of electrical sensors, optical sensors, acoustic sensors, and combinations thereof; and/or
(ii) comprises a material that is selected from the group consisting of metals, semiconductors, electrically conductive polymers, and combinations thereof.

3. The apparatus of claim 1 , wherein the at least one well is constructed from a material that
(i) is selected from the group consisting of metals, plastics, and combinations thereof; and/or
(ii) is transparent for light with a wavelength in the range from 300 nm to 700 nm; and/or
(iii) is sterilized; and/or
(iv) is hydrophilized by an argon plasma at least regionally.

4. The apparatus of claim 1 , wherein the base of the at least one well is provided with an elevated portion that is connected to the base of the at least one well in a fluid-tight manner, with the elevated portion
(i) having a height that is less than a height of the at least one well and/or
(ii) bounds a surface at the base of the at least one well that is smaller than the total surface of the base of the at least one well.

5. The apparatus of claim 1 , wherein the at least one well is at least one well of a multiwell plate and the multiwell plate includes at least one further well.

6. The apparatus of claim 1 , wherein the medium for cryopreservation includes
(i) an aqueous buffer solution; and/or
(ii) a cryoprotective substance selected from the group consisting of DMSO, glycerin, trehalose, and mixtures thereof.

Assignees

• Fraunhofer Ges Forschung
• Univ Regensburg

Inventors

• Wegener Joachim
• Michaelis Stefanie

Classifications

• A01N1/147

  Carriers for immersion in cryogenic fluid for slow freezing or vitrification · CPC title

• B01L3/50851 Primary

  specially adapted for heating or cooling samples · CPC title

• A01N1/0268

  Human Necessities · mapped topic

• C12N1/04

  Preserving or maintaining viable microorganisms (immobilised microorganisms C12N11/00) · CPC title

• B01L2300/0645

  Electrodes · CPC title