Microdevice for detecting aldehydes or ketones

Patent metadata

Field	Value
Publication number	US-11969729-B2
Application number	US-202017264267-A
Country	US
Kind code	B2
Filing date	Apr 16, 2020
Priority date	Apr 19, 2019
Publication date	Apr 30, 2024
Grant date	Apr 30, 2024

Abstract

A microdevice for detecting aldehydes or ketones by utilizing a rotary platform, the microdevice comprising a disc-shaped rotary platform and a microfluidic structure disposed on the rotary platform, and the microfluidic structure comprising a sample storage part, an eluent storage part, a separation part, a first microfluid flow channel, a second microfluid flow channel, and an absorption pad.

First claim

What is claimed is:

1. A device for detecting aldehydes or ketones, comprising:
a rotary platform of a disk shape; and
a microfluidic structure disposed on the rotary platform, wherein the microfluidic structure includes:
a sample storage unit into which a fluid sample containing aldehydes or ketones can be injected to derivatize the aldehydes or the ketones;
an eluent storage unit into which an eluent can be injected;
a separation unit having a thin layer chromatography plate configured to receive the sample from the sample storage unit and the eluent from the eluent storage unit, to separate the aldehydes or the ketones of the sample and to deploy the aldehydes or the ketones of the sample with the eluent;
a first microfluidic channel configured to move the sample to the separation unit, and which connects the sample storage unit and the separation unit;
a second microfluidic channel configured to move the eluent to the separation unit, and which connects the eluent storage unit and the separation unit; and
an absorption pad configured to receive the eluent from the eluent storage unit and to discharge the eluent to the thin layer chromatography plate, wherein the separation unit includes a sample introduction portion for receiving the sample from the sample storage unit;
an eluent introduction portion for receiving the eluent from the eluent storage unit; and
a deployment portion configured to separate the aldehydes or the ketones of the sample and to deploy the aldehydes or the ketones of the sample with the eluent, and the absorption pad is provided in the eluent introduction portion, wherein the center of rotation at which a rotational axis of the rotary platform provided in the rotary platform is the center of the rotary platform, a longitudinal direction of the separation unit is a radial direction of the rotary platform, and the deployment portion is a position farther from the center of rotation than the sample introduction portion and the eluent introduction portion are from the center of rotation, wherein the rotary platform is configured to rotate in a first direction perpendicular to a surface of the rotary platform in a rotational direction of the rotational axis, the thin layer chromatography plate on which the eluent can be deployed by a capillary force is disposed across the deployment portion and the eluent introduction portion in the separation unit such that a longitudinal direction of the thin layer chromatography plate points in a direction of a centrifugal force generated by a rotation, and the eluent can be discharged from the eluent introduction portion to the deployment portion, and can be propelled in the deployment portion by a combined force of the capillary force and the centrifugal force, wherein the first microfluidic channel comprises one or more first microfluidic bent portions and the second microfluidic channel comprises two or more second microfluidic bent portions and wherein a number of the two or more second microfluidic bent portions of the second microfluidic channel is more than a number of the one or more first microfluidic bent portions of the first microfluidic channel.

2. The device according to claim 1 ,
wherein a first end of the first microfluidic channel is connected to the sample storage unit and a second end of the first microfluidic channel is connected to the sample introduction portion, and
a first end of the second microfluidic channel is connected to the eluent storage unit and a second end of the second microfluidic channel is connected to the eluent introduction portion.

3. The device according to claim 1 ,
wherein the absorption pad is made of a cellulose fiber, a gelatin fiber, a starch fiber or a mixture of two or more thereof.

4. The device according to claim 1 , wherein the aldehydes or the ketones contained in the sample includes at least one selected from the group consisting of an acetaldehyde, an acetone, an acrolein, a benzaldehyde, a butyraldehyde, a formaldehyde, and a propionaldehyde.

5. The device according to claim 1 ,
wherein the inside of the sample storage unit is filled with 2,4-dinitrophenylhydrazine coated silica (2, 4-DNPH-coated silica) in the form of beads.

6. The device according to claim 1 ,
wherein the microfluidic structure is provided in plurality, and the plurality of microfluidic structures are capable of accommodating different fluid samples from each other, respectively, and are disposed radially symmetrically on the rotary platform.

7. The device according to claim 1 , wherein the microfluidic structure further includes a first vent hole and a second vent hole through which an external gas can be injected into the separation unit or an internal gas inside the separation unit can be discharged to the external; a first air circulation channel configured to move gas between the first vent hole and the separation unit; and a second air circulation channel configured to move gas between the second vent hole and the separation unit, and wherein the first air circulation channel is connected to one end of the separation unit, and the second air circulation channel is connected to the other end of the separation unit.

8. The device according to claim 1 , wherein the microfluidic structure further includes a waste channel for isolating a part of the sample moving from the sample storage unit to the separation unit, and wherein the waste channel branches from the first microfluidic channel.

Assignees

• Lg Chemical Ltd

Inventors

• Park Byung Hyun
• Kim Byoung Hyoun
• Han Su Youn

Classifications

• B01L3/50273 Primary

  characterised by the means or forces applied to move the fluids · CPC title

• B01L3/502723 Primary

  characterised by venting arrangements · CPC title

• B01L3/502753

  characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation · CPC title

• G01N30/92

  Construction of the plate · CPC title

• B01L2200/0652

  Sorting or classification of particles or molecules · CPC title