Recombinant microorganism transformed with a glutaric acid transporter gene and method of preparing glutaric acid using same

Patent metadata

Field	Value
Publication number	US-11965200-B2
Application number	US-202117549757-A
Country	US
Kind code	B2
Filing date	Dec 13, 2021
Priority date	Dec 24, 2020
Publication date	Apr 23, 2024
Grant date	Apr 23, 2024

Abstract

The present invention relates to a recombinant microorganism imparted with increased ability to produce glutaric acid by further introducing a gene encoding a polypeptide having glutaric acid transporter activity into a microorganism having ability to produce glutaric acid, and to a method of preparing glutaric acid using the recombinant microorganism. According to the present invention, glutaric acid used for the preparation of various compounds such as polyamide, polyurethane, 1,5-pentanediol, and 5-hydroxyvaleric acid can be biosynthesized at high yield.

First claim

The invention claimed is:

1. A recombinant Corynebacterium glutamicum that produces glutaric acid, wherein the Corynebacterium glutamicum is transformed with the Corynebacterium glutamicum ddh gene, the Corynebacterium glutamicum gabT gene, the Corynebacterium glutamicum gabD gene, the Pseudomonas putida davA gene and Pseudomonas putida davB gene,
in which the Corynebacterium glutamicum lysE gene is deleted, and
in which the Corynebacterium glutamicum ynfM gene of SEQ ID NO: 2 encoding a polypeptide having glutaric acid transporter activity is expressed under a strong heterologous promoter.

2. A method of preparing glutaric acid, comprising:
(a) producing glutaric acid by culturing the recombinant microorganism according to claim 1 ; and
(b) recovering the produced glutaric acid.

Assignees

• Korea Advanced Inst Sci & Tech

Inventors

• Lee Sang Yup
• Han Tae Hee

Classifications

• C12P7/44 Primary

  Polycarboxylic acids · CPC title

• C12N15/77 Primary

  for Corynebacterium; for Brevibacterium · CPC title

• C12R2001/15

  Corynebacterium · CPC title

• C12N9/00 Primary

  Enzymes; Proenzymes; Compositions thereof (preparations containing enzymes for cleaning teeth A61K8/66, A61Q11/00; medicinal preparations containing enzymes or proenzymes A61K38/43; enzyme containing detergent compositions C11D; {enzymes with nucleic acid structure, e.g. ribozymes, C12N15/113}); Processes for preparing, activating, inhibiting, separating or purifying enzymes (preparation of malt C12C1/00) · CPC title

• C12N15/52

  Genes encoding for enzymes or proenzymes · CPC title