Enzymatic preparation method of inclusion complexes of tributyrin

Patent metadata

Field	Value
Publication number	US-11965196-B2
Application number	US-202017034234-A
Country	US
Kind code	B2
Filing date	Sep 28, 2020
Priority date	Apr 18, 2018
Publication date	Apr 23, 2024
Grant date	Apr 23, 2024

Abstract

The disclosure relates to an enzymatic preparation method of inclusion complexes of tributyrin, and belongs to the technical field of oil microencapsulation. The disclosure combines enzymatic synthesis of cyclodextrin and inclusion of tributyrin with cyclodextrin, including enzymatic preparation of cyclodextrin with a CGT enzyme. Tributyrin is added in the preparation process; after reaction, Tween is added, and homogenization and spray drying are carried out. The effect of the finally obtained tributyrin powder is much better than that of single inclusion of tributyrin with cyclodextrin. The disclosure is simple in process, low in cost and convenient in operation; reaction processes are free of toxicity and pollution; there are no toxic reagent residues; the inclusion effect is obvious, and better utilization of a nutritional additive tributyrin in actual production is facilitated.

First claim

What is claimed is:

1. A method of enzymatic preparation of inclusion complexes of tributyrin, comprising:
(a) preparing starch milk or a maltodextrin solution as a raw material, wherein a mass concentration of the starch milk or maltodextrin solution is 15% to 30%, and wherein the starch milk is an aqueous suspension of starch from one or more of corn starch, waxy corn starch, potato starch, rice starch, cassava starch, and wheat starch;
(b) adding an enzyme into the starch milk or maltodextrin solution for liquefaction reaction and cyclization reaction to form cyclodextrin, wherein the enzyme is cyclodextrin glucosyltransferase which catalyzes cyclization of glucan polymers in the starch milk to form the cyclodextrin, and wherein liquefaction is performed at a temperature of 80° C. to 90° C.; and
(c) adding tributyrin and an emulsifier so that tributyrin is included,
wherein there is no separation and/or purification of the cyclodextrin between steps (b) and (c).

2. The method according to claim 1 , wherein maltodextrin in the maltodextrin solution is maltodextrin from a starch source with a dextrose equivalent (DE) value of 5 to 25.

3. The method according to claim 1 , further comprising: (d) mixing, (e) shearing, (r) homogenizing, and (g) spray drying.

4. The method according to claim 1 , wherein a mass ratio of tributyrin to starch in the starch milk or maltodextrin in the maltodextrin solution is (0.30-0.75):1.

5. The method according to claim 1 , wherein in the liquefaction reaction, an addition amount of cyclodextrin glucosyltransferase is 2 to 4 Unit/gram starch or maltodextrin wet substrate, and the liquefaction reaction time is 30 to 60 minutes.

6. The method according to claim 1 , wherein in the cyclization reaction, an addition amount of cyclodextrin glucosyltransferase is 2 to 4 Unit/gram starch or maltodextrin wet substrate, a cyclization temperature is 45° C., and the cyclization reaction time is 8 to 12 hours.

7. The method according to claim 1 , wherein the emulsifier is any one or more of Tween, arabic gum and xanthan gum, and a mass ratio of the emulsifier to starch in the starch milk or maltodextrin in the maltodextrin solution is (0.3-0.5):1.

8. The method according to claim 3 , wherein a shearing speed is 8000 rpm to 20000 rpm, and the shearing time is 2 to 5 minutes; a homogenization pressure is 30 MPa to 40 MPa, and a homogenization frequency is 2 to 3; and spray drying conditions are under: an air inlet temperature is 180° C. to 185° C., an air outlet temperature is 85° C. to 95° C., and a feeding flow rate is 20 mL/min to 22 mL/min.

Assignees

• Univ Jiangnan

Inventors

• Li Caiming
• Li Zhaofeng
• Chen Shuangdi
• Gu Zhengbiao
• Feng Yan
• Li Zexi
• Cheng Li
• Hong Yan

Classifications

• C12P19/04 Primary

  Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds · CPC title

• A23K10/14

  Pretreatment of feeding-stuffs with enzymes · CPC title

• A23K20/158

  Fatty acids; Fats; Products containing oils or fats · CPC title

• A23K20/163

  Sugars; Polysaccharides · CPC title

• A23K40/30

  by encapsulating; by coating · CPC title