Liquid chromatography system and component
US-11131020-B2 · Sep 28, 2021 · US
Methods to increase sensitivity of LC/MS analysis
US11959842B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11959842-B2 |
| Application number | US-202217990029-A |
| Country | US |
| Kind code | B2 |
| Filing date | Nov 18, 2022 |
| Priority date | Jan 17, 2020 |
| Publication date | Apr 16, 2024 |
| Grant date | Apr 16, 2024 |
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- Title
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Abstract
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The present disclosure discusses a method of separating a sample (e.g., pharmaceutical drug, genotoxic impurity, biomarker, and/or biological metabolite) including coating a metallic flow path of a chromatographic system; injecting the sample into the chromatographic system; flowing the sample through the chromatographic system; separating the sample; and analyzing the separated sample using mass spectroscopy. In some examples, the coating applied to the surfaces defining the flow path is non-binding with respect to the sample—and the separated sample. Consequently, the sample does not bind to the low-binding surface of the coating of the flow path. The applied coating can increase the chromatographic peak area for the sample of the chromatographic system.
First claim
Opening claim text (preview).
What is claimed is: 1. A method of separating a sample comprising: providing a chromatographic system having a metallic flow path with an alkylsilyl coating on fluid exposed surfaces, the metallic flow path with the alkylsilyl coating preventing the sample from binding to underlying metal surfaces; injecting the sample into the chromatographic system, wherein the sample comprises gentisic acid and/or 4-hydroxybenzoic acid; flowing the sample through the chromatographic system; and separating the sample, wherein coating the metallic flow path of the chromatographic system increases a chromatographic peak area of the gentisic acid and/or 4-hydroxybenzoic acid in the sample in comparison to an uncoated chromatographic system having metallic surfaces exposed to fluid by 1.3 × or greater. 2. The method of claim 1 , wherein the alkylsilyl coating is applied to internal portions of the column, frits and end caps. 3. The method of claim 1 , wherein the alkylsilyl coating is applied to wetted surfaces located upstream of the chromatographic column. 4. The method of claim 1 , wherein the chromatographic system comprises a mix-mode chromatographic column. 5. The method of claim 1 , wherein the chromatographic system comprises a phenyl-hexyl chromatographic column. 6. The method of claim 1 , wherein the alkylsilyl coating comprises bis(trichlorosilyl)ethane or bis(trimethoxysilyl)ethane.
Assignees
Inventors
Classifications
- G01N1/405Primary
by adsorption or absorption · CPC title
interfaced to liquid or supercritical fluid chromatograph (interfaces in general for introducing or extracting samples to be analysed with specially adapted mass spectrometer, see H01J49/04) · CPC title
Peaks · CPC title
Liquid chromatography · CPC title
- G01N30/56Primary
Packing methods or coating methods · CPC title
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- What does patent US11959842B2 cover?
- The present disclosure discusses a method of separating a sample (e.g., pharmaceutical drug, genotoxic impurity, biomarker, and/or biological metabolite) including coating a metallic flow path of a chromatographic system; injecting the sample into the chromatographic system; flowing the sample through the chromatographic system; separating the sample; and analyzing the separated sample using ma…
- Who is the assignee on this patent?
- Waters Technologies Corp
- What technology area does this patent fall under?
- Primary CPC classification G01N1/405. Mapped technology areas include Physics.
- When was this patent published?
- Publication date Tue Apr 16 2024 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 7 related publications on this page (citations in our corpus or others sharing the same primary CPC).