Photoactive fluorophores and methods of in vivo labeling

Patent metadata

Field	Value
Publication number	US-11958976-B2
Application number	US-202217975310-A
Country	US
Kind code	B2
Filing date	Oct 27, 2022
Priority date	May 20, 2016
Publication date	Apr 16, 2024
Grant date	Apr 16, 2024

Abstract

Provided are a photoactive fluorophore, a photoactive ligand, and a photoactive complex. The photoactive fluorophore includes a photoactivatable derivative of an azetidine-containing Janelia-Fluor dye. The photoactive ligand includes a photoactive fluorophore and a protein tag. The photoactive complex includes a photoactive ligand conjugated to a protein. Also provided are methods of in vivo labeling with and photoactivation of the photoactive fluorophore, ligand, and complex.

First claim

What is claimed is:

1. A compound of the formula:

wherein R is selected from the group consisting of a ligand that is a protein tag, and N-hydroxysuccinimide (NHS) ester.

2. A photoactive complex, comprising the compound according to claim 1 wherein R is the ligand, conjugated to a protein that binds the ligand.

3. The photoactive complex of claim 2 , wherein the compound is conjugated to the protein in vivo.

4. The compound of claim 1 , wherein the ligand is selected from the group consisting of:

5. The compound of claim 1 , of the formula:

6. The compound of claim 1 , of the formula:

7. The compound of claim 1 , of the formula:

Assignees

• Hughes Howard Med Inst

Inventors

• Lavis Luke D
• Grimm Jonathan B

Classifications

• C09B11/24 Primary

  Phthaleins containing amino groups {; Phthalanes; Fluoranes; Phthalides; Rhodamine dyes; Phthaleins having heterocyclic aryl rings; Lactone or lactame forms of triarylmethane dyes} · CPC title

• C07D311/80

  Dibenzopyrans; Hydrogenated dibenzopyrans · CPC title

• C07D313/14

  [b,f]-condensed · CPC title

• C07D405/12

  linked by a chain containing hetero atoms as chain links · CPC title

• C07D473/32

  Nitrogen atom · CPC title