Extracellular potential measurement device

Patent metadata

Field	Value
Publication number	US-11946847-B2
Application number	US-202017772219-A
Country	US
Kind code	B2
Filing date	Oct 1, 2020
Priority date	Nov 14, 2019
Publication date	Apr 2, 2024
Grant date	Apr 2, 2024

Abstract

An extracellular potential measurement device includes multiple insulating films each of which is made from an electric insulating material, the insulating films being stacked and bonded to each other; and multiple electrode wires each of which is made from an electroconductive material, the electrode wires being arranged in multiple heights. Each of the electrode wires is interposed between an upper insulating film and a lower insulating film. Each of the insulating films, except for a lowermost insulating film, has an opening penetrating the insulating film. The opening in a lower insulating film has a size that is less than that of the opening in an upper insulating film, the openings in the insulating films being overlapped to form a recess having a size reducing downward, the recess being adapted to store a collection of cells. Each of the electrode wires has an end that is located near an opening in an insulating film that is immediately below the electrode wire, the ends being exposed in the recess.

First claim

The invention claimed is:

1. An extracellular potential measurement device comprising:
multiple insulating films each of which is made from an electric insulating material, the insulating films being stacked and bonded to each other; and
multiple electrode wires each of which is made from an electroconductive material, the electrode wires being arranged in multiple heights,
each of the electrode wires being interposed between an upper insulating film and a lower insulating film,
each of the insulating films, except for a lowermost insulating film, having an opening penetrating the insulating film,
the opening in a lower insulating film having a size that is less than that of the opening in an upper insulating film, the openings in the insulating films being overlapped to form a recess having a size reducing downward, the recess being adapted to store a collection of cells,
each of the electrode wires having an end that is located near an opening in an insulating film that is immediately below the electrode wire, the ends being exposed in the recess.

2. The extracellular potential measurement device according to claim 1 , further comprising an electrode wire made from an electric insulating material and interposed between the lowermost insulating film and a second lowermost insulating film, wherein an end of the electrode wire interposed between the lowermost insulating film and the second lowermost insulating film traverses the opening of the second lowermost insulating film entirely in one direction.

3. The extracellular potential measurement device according to claim 2 , wherein an uppermost insulating film has a groove penetrating the uppermost insulating film, the groove being adapted to store a linear axon extending from the collection of cells, the groove being connected to the recess,
the extracellular potential measurement device further comprising at least one axon electrode wire, an end of the axon electrode wire is exposed in the groove so as to be brought into contact with the axon.

4. The extracellular potential measurement device according to claim 3 , further comprising multiple second electrode wires each of which is made from an electroconductive material, the second electrode wires being arranged in multiple heights,
each of the second electrode wires being interposed between an upper insulating film and a lower insulating film,
each of the insulating films, except for the lowermost insulating film, having a second opening penetrating the insulating film,
the second opening in a lower insulating film having a size that is less than that of the second opening in an upper insulating film, the second openings in the insulating films being overlapped to form a second recess having a size reducing downward, the second recess being adapted to store a second collection of cells to which the axon is connected, the second recess being connected to the groove,
each of the second electrode wires having an end that is located near a second opening in an insulating film that is immediately below the second electrode wire, the ends of the second electrode wires being exposed in the second recess.

5. The extracellular potential measurement device according to claim 1 , wherein an uppermost insulating film has a groove penetrating the uppermost insulating film, the groove being adapted to store a linear axon extending from the collection of cells, the groove being connected to the recess,
the extracellular potential measurement device further comprising at least one axon electrode wire, an end of the axon electrode wire is exposed in the groove so as to be brought into contact with the axon.

6. The extracellular potential measurement device according to claim 5 , further comprising multiple second electrode wires each of which is made from an electroconductive material, the second electrode wires being arranged in multiple heights,
each of the second electrode wires being interposed between an upper insulating film and a lower insulating film,
each of the insulating films, except for the lowermost insulating film, having a second opening penetrating the insulating film,
the second opening in a lower insulating film having a size that is less than that of the second opening in an upper insulating film, the second openings in the insulating films being overlapped to form a second recess having a size reducing downward, the second recess being adapted to store a second collection of cells to which the axon is connected, the second recess being connected to the groove,
each of the second electrode wires having an end that is located near a second opening in an insulating film that is immediately below the second electrode wire, the ends of the second electrode wires being exposed in the second recess.

7. The extracellular potential measurement device according to claim 1 , wherein the upper insulating film and the lower insulating film are bonded together with an adhesive.

8. An extracellular potential measurement device comprising:
a sheet comprising multiple insulating films each of which is made from an electric insulating material, the insulating films being stacked and bonded to each other, the sheet further comprising a recess adapted to store a collection of cells and a groove connected to the recess, the groove being adapted to store a linear axon extending from the collection of cells; and
multiple electrode wires interposed between the insulating films of the sheet,
multiple electrode wires having ends exposed in the recess so as to be brought into contact with the collection of cells,
at least one electrode wire having an end exposed in the groove so as to be brought into contact with the axon.

9. The extracellular potential measurement device according to claim 8 , wherein the electrode wires that have ends exposed in the recess are arranged in multiple heights.

10. The extracellular potential measurement device according to claim 9 , wherein each of the insulating films, except for a lowermost insulating film, has an opening penetrating the insulating film,
the opening in a lower insulating film having a size that is less than that of the opening in an upper insulating film, the openings in the insulating films being overlapped to form the recess having a size reducing downward,
each of the ends of the electrode wires exposed in the recess being located near an opening in an insulating film that is immediately below the electrode wire.

11. The extracellular potential measurement device according to claim 8 , further comprising multiple second electrode wires interposed between the insulating films of the sheet,
the sheet comprising a second recess adapted to store a second collection of cells to which the axon is connected, the second recess being connected to the groove,
each of the second electrode wires having an end exposed in the second recess so as to be brought into contact with the second collection of cells.

12. The extracellular potential measurement device according to claim 11 , wherein the second electrode wires are arranged in multiple heights.

13. The extracellular potential measurement device according to claim 12 , wherein each of the insulating films, except for a lowermost insulating film, has a second opening penetrating the insulating film,
the second opening in a lower insulating film having a size that is less than that of the second opening in an upper insulating film, the second openings in the insulating films being overlapped to form the second recess having a size reducing downward,
each of the second electrode wires having an end that is located near a second opening in an insulating film th

Assignees

• Nok Corp
• Univ Tokyo

Inventors

• Komori Takayuki
• Miyajima Keiichi
• Kim Soohyeon
• Fujii Teruo
• Okawa Shinji

Classifications

• G01N15/1031 Primary

  by measuring electrical or magnetic effects · CPC title

• C12M41/28

  of redox potential · CPC title

• G01N2015/0065

  Physics · mapped topic

• G01N33/48728 Primary

  Investigating individual cells, e.g. by patch clamp, voltage clamp (investigating individual particles in general G01N15/10) · CPC title

• C12M41/46

  of cellular or enzymatic activity or functionality, e.g. cell viability · CPC title