Bacillus gibsonii-clade serine proteases

Patent metadata

Field	Value
Publication number	US-11946081-B2
Application number	US-201716471686-A
Country	US
Kind code	B2
Filing date	Dec 19, 2017
Priority date	Dec 21, 2016
Publication date	Apr 2, 2024
Grant date	Apr 2, 2024

Abstract

Disclosed herein is one or more subtilisin variant. nucleic acid encoding same. and compositions and methods related to the production and use thereof. including one or more Bacillus gibsonii-clade subtilisin variant that has improved soil removal compared to one or more reference subtilisin.

First claim

We claim:

1. A subtilisin variant comprising an amino acid sequence that is at least 90% identical to the amino acid sequence set forth in SEQ ID NO: 1, wherein the variant has the substitutions 5039E-5099R-S126A-D127E-F128G numbered by correspondence with the amino acid sequence of SEQ ID NO:1, and wherein the variant has a blood/milk/ink on cotton (BMI), egg, and/or crème brûlée stain cleaning PI>1 when compared to SEQ ID NO:1.

2. The subtilisin variant of claim 1 , wherein the variant has combinations of mutations selected from the group consisting of:
S039E-S099R-S126A-D127E-F128G-M211L, 5039E-S099R-S126A-D127E-F128G-N242D, 5039E-S099R-S126A-D127E-F128G-N253D, 5039E-S099R-S126A-D127E-F128G-Q200L, 5039E-S099R-S126A-D127E-F128G-Q256E, 5039E-S099R-S126A-D127E-F128G-S255W, 5039E-5099R-S126A-D127E-F128G-V199I, S039E-5099R-S126A-D127E-F128G-Y203W, 5039E-5099R-T1140-S126A-D127E-F128G, 5039E-T056Y-N074D-S099R-S126A-D127E-F128G, 5039E-T056Y-S099R-S126A-D127E-F128G, 5039E-T056Y-S099R-S126A-D127E-F128G-M211L, and T009E-5039E-5099R-S126A-D127E-F128G, numbered by correspondence with the amino acid sequence of SEQ ID NO:1.

3. The subtilisin variant of claim 1 , wherein the variant comprises an amino acid sequence with 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or less than 100% amino acid sequence identity to the amino acid sequence of SEQ ID NO:1.

4. A composition comprising the substilisin variant of claim 1 .

5. The composition of claim 4 , wherein the composition is selected from an enzyme composition and a detergent composition.

6. The composition of claim 5 , wherein the detergent composition is selected from a laundry detergent, a fabric softening detergent, a dishwashing detergent, and a hard-surface cleaning detergent.

7. The composition of claim 4 , further comprising (i) one or more enzymes selected from acyl transferases, amylases, alpha-amylases, beta-amylases, alpha-galactosidases, arabinases, arabinosidases, aryl esterases, beta-galactosidases, beta-glucanases, carrageenases, catalases, chondroitinases, cutinases, endo-beta-mannanases, exo-beta-mannanases, esterases, exo-mannanases, galactanases, glucoamylases, hemicellulases, hyaluronidases, keratinases, lactases, ligninases, lipases, lipolytic enzymes, lipoxygenases, mannanases, metalloproteases, oxidases, oxidoreductases, pectate lyases, pectin acetyl esterases, pectinases, pentosanases, perhydrolases, peroxidases, phenoloxidases, phosphatases, phospholipases, polyesterases, polygalacturonases, proteases, pullulanases, reductases, rhamnogalacturonases, cellulases, beta-glucanases, tannases, transglutaminases, xylan acetyl-esterases, xylanases, and xylosidases; (ii) one or more surfactants; (iii) one or more ions selected from calcium and zinc; (iv) one or more adjunct material; (v) one or more stabilizers; (vi) one or more bleaching agents and/or (vii) combinations thereof.

8. The composition of claim 4 , wherein the composition is phosphate-free or contains phosphate and/or is boron free or contains boron.

9. The composition of claim 4 , wherein the composition is granular, powder, solid, bar, liquid, tablet, gel, paste and/or a unit dose composition.

Assignees

• Danisco Us Inc

Inventors

• Babe Lilia Maria
• Dankmeyer Lydia
• Goedegebuur Frits
• Kaper Thijs
• Mulder Harm
• Pricelius Sina

Classifications

• C12N9/54 Primary

  bacteria being Bacillus · CPC title

• C11D3/38681

  Chemically modified or immobilised enzymes · CPC title

• C12Y304/21062

  Subtilisin (3.4.21.62) · CPC title

• C11D3/386

  Preparations containing enzymes {, e.g. protease or amylase} · CPC title