Bacterium and obtaining method and application thereof

Patent metadata

Field	Value
Publication number	US-11946036-B2
Application number	US-202217671001-A
Country	US
Kind code	B2
Filing date	Feb 14, 2022
Priority date	Jul 15, 2016
Publication date	Apr 2, 2024
Grant date	Apr 2, 2024

Abstract

The present invention discloses a bacterium and an obtaining method and application thereof. The bacterium has a property of coproducing 1,3-propanediol and D-lactic acid. Further, the bacterium is Klebsiella oxytoca , including Klebsiella oxytoca PDL-5 CCTCC M 2016185. The obtaining method of the bacterium may be to obtain the bacterium by directly screening wild bacteria that satisfy conditions from the environment or performing gene engineering modification to wild bacteria. The present invention has the advantages that the bacteria can coproduce 1,3-propanediol and D-lactic acid through fermentation, the molar conversion rate and the concentration of the two products are very high, the types of byproducts are few, the concentration is low, the product extraction process is simplified, the high-efficiency biological production of 1,3-propanediol and D-lactic acid can be realized, and the industrial application prospect is very great.

First claim

The invention claimed is:

1. An artificial bacterium, wherein the artificial bacterium is Klebsiella oxytoca PDL-0 with genes budA and budB defects, or
the artificial bacterium is Klebsiella oxytoca PDL-0 with genes budA, budB and adhE defects; or
the artificial bacterium is Klebsiella oxytoca PDL-0 with genes budA, budB, adhE and ackA-pta defects; or
the artificial bacterium is Klebsiella oxytoca PDL-0 with genes budA, budB, adhE, ackA-pta and poxB defects; or
the artificial bacterium is Klebsiella oxytoca PDL-0 with genes budA, budB, adhE, ackA-pta, poxB and frdA defects, and
wherein the Klebsiella oxytoca PDL-0 was collected in China Center for Type Culture Collection on Apr. 8, 2016, and the collection registration number is CCTCC M 2016184.

2. The artificial bacterium according to claim 1 , wherein a DNA sequence of the budA is shown in SEQ ID NO:1; and/or a DNA sequence of the budB is shown in SEQ ID NO:2; and/or a DNA sequence of the adhE is shown in SEQ ID NO:3; and/or a DNA sequence of the ackA-pta is shown in SEQ ID NO:4; and/or a DNA sequence of the poxB is shown in SEQ ID NO:5; and/or a DNA sequence of the frdA is shown in SEQ ID NO:6.

3. The artificial bacterium according to claim 1 , wherein the artificial bacterium has a property of coproducing 1,3-propanediol and D-lactic acid and in products obtained through coproduction, a total conversion rate of 1,3-propanediol and D-lactic acid exceeds 90%.

4. The artificial bacterium according to claim 1 , wherein the artificial bacterium has the following metabolic pathways:
1) glycerol→1,3-propanediol; and
2) glycerol→pyruvic acid→D-lactic acid.

Assignees

• Univ Shanghai Jiaotong

Inventors

• Xu Ping
• Xin Bo
• Tao Fei
• Wang Yu
• Tang Hongzhi
• Ma Cuiqing

Classifications

• C12N1/20 Primary

  Bacteria; Culture media therefor · CPC title

• C12N9/0006 Primary

  acting on CH-OH groups as donors (1.1) · CPC title

• C12N9/0008

  acting on the aldehyde or oxo group of donors (1.2) · CPC title

• C12N9/001

  acting on the CH-CH group of donors (1.3) · CPC title

• C12N9/1022

  transferring aldehyde or ketonic groups (2.2) · CPC title