Systems and methods for monitoring behavior of cells and/or tissues in culturing media

Patent metadata

Field	Value
Publication number	US-11946027-B2
Application number	US-202016883427-A
Country	US
Kind code	B2
Filing date	May 26, 2020
Priority date	May 24, 2019
Publication date	Apr 2, 2024
Grant date	Apr 2, 2024

Abstract

Systems and methods for monitoring and tracking the behavior of cells and tissues in a culturing medium, for example, in a cell culture medium or an in vivo tissue environment, using a tissue scaffold system having one or more sensor arrays. Such monitoring systems include a tissue scaffold system and one or more sensor arrays on the tissue scaffold system in vertically stackable configurations. The sensor array(s) are configured to monitor electrical impedance and/or electrophysiological activities of cells or tissues which may be provided to an external data acquisition system for production of a three-dimensional (3D) map.

First claim

The invention claimed is:

1. A buoyant instrumented tissue scaffold system for sensing behaviors and/or functions of cells or tissues in a culturing medium, the system comprising:
a buoyant member comprising a sheet of hydrophobic microporous polydimethylsiloxane (PDMS) coated with a surface tension increasing covering, the sheet having an opening through a central portion thereof;
a scaffold supported on the buoyant member and disposed in the opening, the buoyant member being anchored to a peripheral area of the scaffold, the scaffold comprising one or more flexible members and one or more sensor arrays on the one or more flexible members, the one or more sensor arrays being configured to sense the behaviors and/or functions of the cells or tissues in the culturing medium; and
a transmitting means functionally coupled to the one or more sensor arrays for transmitting information obtained thereby to an external data acquisition unit;
wherein the buoyant member maintains the scaffold and the transmitting means afloat on a surface of the culturing medium, enables the cells or tissues to reside and grow beneath the scaffold, and is a barrier to wetting of the one or more sensor arrays by the culturing medium.

2. The buoyant instrumented tissue scaffold system of claim 1 , wherein the system comprises a plurality of the scaffold in a stacked configuration, and each of the scaffolds comprises one or more of the one or more sensor arrays on one or more of the flexible members.

3. The buoyant instrumented tissue scaffold system of claim 2 , the system further comprises means for producing a three dimensional (3D) map based on information received from the one or more sensor arrays.

4. The buoyant instrumented tissue scaffold system of claim 1 , wherein the behaviors and/or functions of the cells or tissues sensed by the one or more sensor arrays comprise at least one of cellular electrical impedance and cellular electrophysiological signals of the cells or tissues.

5. The buoyant instrumented tissue scaffold system of claim 1 , wherein at least one sensor of the one or more sensor arrays senses pH, pressure, temperature, and/or mechanical strains.

6. The buoyant instrumented tissue scaffold system of claim 1 , wherein the culturing medium is a cell culture medium.

7. The buoyant instrumented tissue scaffold system of claim 1 , wherein the culturing medium is an in vivo tissue environment.

8. The buoyant instrumented tissue scaffold system of claim 1 , wherein the scaffold comprises a waterproof encapsulation layer that encases the one or more sensor arrays.

9. The buoyant instrumented tissue scaffold system of claim 1 , wherein the transmitting means comprises flexible conductors connecting the one or more sensor arrays with the external data acquisition unit.

10. The buoyant instrumented tissue scaffold system of claim 1 , wherein the buoyant member is a microporous water-repellent film and the surface tension increasing covering comprises polytetrafluorethylene nanoparticles mixed with polytetrafluorethylene.

11. The buoyant instrumented tissue scaffold system of claim 1 , wherein the scaffold comprises thin film electrodes disposed on a first layer of polyimide and encapsulated with a second layer of polyimide.

12. The buoyant instrumented tissue scaffold system of claim 1 , wherein the one or more flexible members encapsulate the one or more sensor arrays.

13. The buoyant instrumented tissue scaffold system of claim 12 , wherein the one or more flexible members are formed of an elastomeric polyimide.

14. The buoyant instrumented tissue scaffold system of claim 1 , wherein the culturing medium surrounds the scaffold.

15. A method of sensing behaviors and/or functions of cells or tissues in a culturing medium using the buoyant instrumented tissue scaffold system of claim 1 , the method comprising:
locating the scaffold on the culturing medium such that the buoyant member floats on or in the culturing medium and is a barrier to wetting of the sensor arrays by the culturing medium;
allowing the cells or tissues to reside and/or grow beneath the scaffold;
detecting the behaviors and/or functions of the cells or tissues with the sensor arrays; and
transmitting data to the external data acquisition unit corresponding to the behaviors and/or functions.

16. The method of claim 15 , wherein the behaviors and/or functions of the cells or tissues sensed by the sensor arrays comprise at least one of cellular electrical impedance and cellular electrophysiological signals of the cells or tissue.

17. The method of claim 15 , wherein at least one sensor of the sensor arrays senses pH, pressure, temperature, and/or mechanical strains.

18. The method of claim 15 , wherein the culturing medium is a cell culture medium.

19. The method of claim 15 , wherein the culturing medium is an in vivo tissue environment.

20. The method of claim 15 , further comprising producing a three-dimensional (3D) map based on the behaviors and/or functions of the cells or tissues.

Assignees

• Purdue Research Foundation

Inventors

• Lee Chi-Hwan

Classifications

• C12M1/3407 Primary

  Measure of electrical or magnetical factor · CPC title

• C12M21/08 Primary

  for producing artificial tissue or for ex-vivo cultivation of tissue (prostheses A61F2/00, grafts A61L27/00) · CPC title

• C12M23/56

  Floating elements · CPC title

• C12N5/0068

  General culture methods using substrates (for specific animal cell type C12N5/06) · CPC title

• G01N33/4836

  using multielectrode arrays · CPC title