Structure for microbe detection, manufacturing method therefor, and microbe detection method using same structure for microbe detection

Patent metadata

Field	Value
Publication number	US-11940446-B2
Application number	US-201817044878-A
Country	US
Kind code	B2
Filing date	May 16, 2018
Priority date	Apr 3, 2018
Publication date	Mar 26, 2024
Grant date	Mar 26, 2024

Abstract

A method for manufacturing a structure for microbe detection comprises the steps of: reacting nitrilotriacetic acid (NTA) and an acid anhydride to prepare a first compound; chelation of metal ions to the first compound to prepare a second compound; binding the second compound and a microbe detector to prepare a third compound; and mixing an exfoliated transition metal-dichalcogenide (TMD) compound and the third compound to prepare a structure for microbe detection, in which the metal ions of the third compound are bound with the transition metal-dichalcogenide compound.

First claim

The invention claimed is:

1. A method for manufacturing a structure for microbe detection, the method comprising:
reacting nitrilotriacetic acid (NTA) and an acid anhydride to prepare a first compound;
chelation of metal ions to the first compound to prepare a second compound;
binding the second compound and a microbe detector to prepare a third compound; and
mixing an exfoliated transition metal-dichalcogenide (TMD) compound and the third compound to prepare a structure for microbe detection, in which the metal ions of the third compound are bound with the transition metal-dichalcogenide compound.

2. The method of claim 1 , wherein the preparing of the first compound comprises:
dissolving the nitrilotriacetic acid in a solvent;
adding and stirring the acid anhydride and a non-nucleophilic basic material into the solvent in which the nitrilotriacetic acid is dissolved, so as to prepare a mixed solution containing a preliminary first compound;
adding an excessive amount of ester into the mixed solution to precipitate the preliminary first compound of the mixed solution; and
washing and freeze-drying the precipitated preliminary first compound to prepare the first compound.

3. The method of claim 1 , wherein the preparing of the second compound comprises dissolving the first compound in a solvent and adding the metal ions into a resulting solution.

4. The method of claim 3 , wherein the preparing of the second compound is performed at pH in a range of more than 3.7 and less than 6.0.

5. The method of claim 3 , wherein the preparing of the third compound comprises:
adding a cross-linking agent including carbodiimide into the second compound; and
adding and stirring the microbe detector into the second compound to which the cross-linking agent is added.

6. The method of claim 5 , wherein the adding of the cross-linking agent into the second compound comprises adding an activation material of the cross-linking agent in addition to the cross-linking agent.

7. The method of claim 1 , wherein the microbe detector comprises at least one of biomolecules including peptide, DNA or RNA.

Assignees

• Iucf Hyu Erica Campus

Inventors

• Kim Jong-Ho
• Kang Tae Woog
• Lee Sin
• Hwang In Jun
• Han Ju Hee

Classifications

• G01N33/56916 Primary

  Enterobacteria, e.g. shigella, salmonella, klebsiella, serratia · CPC title

• C01B19/00

  Selenium; Tellurium; Compounds thereof (phosphorus compounds C01B25/14) · CPC title

• C01G41/00

  Compounds of tungsten · CPC title

• C01B19/007

  Tellurides or selenides of metals (C01B19/002 takes precedence) · CPC title

• G01N21/6486

  Measuring fluorescence of biological material, e.g. DNA, RNA, cells (G01N21/6428 takes precedence) · CPC title