Continuous automated perfusion culture analysis system (CAPCAS) and applications of same

Patent metadata

Field	Value
Publication number	US-11939563-B2
Application number	US-202217947302-A
Country	US
Kind code	B2
Filing date	Sep 19, 2022
Priority date	Jun 28, 2019
Publication date	Mar 26, 2024
Grant date	Mar 26, 2024

Abstract

A continuous automated perfusion culture analysis system (CAPCAS) comprises one or more fluidic systems configured to operate large numbers of biodevices in parallel. Each fluidic system comprises an input reservoir plate for receiving media; a biodevice plate comprising an array of biodevices fluidically coupled to the input reservoir plate, configured such that each biodevice has independent media delivery, fluid removal, stirring, and gas control, and each biodevice is capable of continuously receiving the media from the input reservoir plate; and an output plate fluidically coupled to the biodevice plate for real-time analysis and sampling. The operations of the CAPCAS are automated and computer-controlled wirelessly. The CAPCAS can also be used for abiotic and biotic chemical synthesis processes.

First claim

What is claimed is:

1. A continuous automated perfusion culture analysis system (CAPCAS), comprising:
one or more fluidic systems configured to operate large numbers of biodevices in parallel,
wherein each fluidic system comprises an array of biodevice perfusion systems and a media delivering means fluidically coupled to the array of biodevices,
wherein the media delivering means comprises a multichannel input selector valve fluidically coupled to input vials, an input pump fluidically coupled to the multichannel input selector valve, and a multichannel input director valve fluidically coupled to the input pump, configured such that the multichannel input selector valve operably selects media and/or drugs from the input vials, and the input director valve allows the input pump to deliver individually the selected media and/or drugs to each biodevice,
wherein the multichannel output collector valve has a connection to pressurized air or other gas to insert one or more bubbles between each sample.

2. The CAPCAS of claim 1 , wherein each fluidic system further comprises a media collecting means, wherein the array of biodevice perfusion systems is fluidically coupled between the media delivering means and the media collecting means.

3. The CAPCAS of claim 2 , wherein the media collecting means comprises a multichannel output collector valve fluidically coupled to the array of biodevices, an output pump fluidically coupled to the multichannel output collector valve, and a multichannel output director valve fluidically coupled to the output pump, configured to remove media from each biodevice and deliver it to waste, a Turbidimeter, a microclinical analyzer, or a holding reservoir.

4. The CAPCAS of claim 3 , wherein the multichannel output collector valve has a connection to back-flush vials, and/or pressurized air or other gas to insert one or more bubbles between each sample.

5. The CAPCAS of claim 3 , further comprising a multichannel reservoir collection valve coupled to the holding reservoir of each fluidic system and configured to analyze media from any single biodevice in any of the one or more fluidic systems.

6. The CAPCAS of claim 5 , further comprising a low-pressure pump fluidically coupled to the multichannel reservoir collection valve for operably withdrawing the media from the holding reservoir that transiently retains the media and cells withdrawn from the desired biodevice or bioreactor well.

7. The CAPCAS of claim 6 , further comprising a bubble detector fluidically coupled to the low pressure pump for operably identifying where one sample ends and another starts, when the low-pressure pump delivers the samples to a mass spectrometer.

8. The CAPCAS of claim 7 , further comprising a calibration valve fluidically coupled to the bubble detector for operably removing air through one port (A), sending leading portions of any sample to waste (W), and injecting either a reagent (R) or a calibration solution (C) into the mass spectrometer.

Assignees

• Univ Vanderbilt

Inventors

• Reiserer Ronald S
• Gerken Gregory B
• Schaffer David K
• Wikswo John P

Classifications

• C12M29/10 Primary

  Perfusion · CPC title

• C12M23/08

  Flask, bottle or test tube · CPC title

• C12M23/10

  Petri dish (crystallising dishes B01L3/06) · CPC title

• C12M23/12

  Well or multiwell plates (C12M25/04 takes precedence) · CPC title

• C12M23/16

  Microfluidic devices; Capillary tubes (integrated microfluidic structures B01L3/5027; microreactors B01J19/0093) · CPC title