Yeast proteins

Patent metadata

Field	Value
Publication number	US-11937620-B2
Application number	US-202318153797-A
Country	US
Kind code	B2
Filing date	Jan 12, 2023
Priority date	Apr 27, 2018
Publication date	Mar 26, 2024
Grant date	Mar 26, 2024

Abstract

The present invention relates to a method for obtaining yeast proteins comprising the following steps: a) providing a yeast cream; b) exposing this yeast cream to a thermal plasmolysis at a temperature between 70 and 95° C. for a time of between 30 seconds and 4 hours; c) subjecting the whole to the activity of at least one ribonuclease and a glucanase, sequentially or simultaneously, at a temperature between 40 and 65° C. for a period of between 8 and 24 hours; and d) separating the insoluble fraction from the soluble fraction; wherein the insoluble fraction collected in step d) is taste-free, has a nucleotide content less than 3% and a true protein content of at least 72%.

First claim

The invention claimed is:

1. A method for obtaining yeast proteins, comprising the following steps:
a) providing a yeast cream;
b) exposing this yeast cream to thermal plasmolysis at a temperature of between 70 and 95° C. for a time of between 30 seconds and 4 hours;
c) subjecting the whole to the activity of at least one ribonuclease and one glucanase, sequentially or simultaneously, at a temperature of between 40 and 65° C. for a time of between 8 and 24 h;
d) separating the insoluble fraction and soluble fraction;
wherein the insoluble fraction collected at step d) is taste-free, has a nucleotide content of less than 3% and true protein content of at least 72%.

2. The use of a yeast protein extract having a true protein content of at least 72%, obtained with the method of claim 1 , in human nutrition or in animal nutrition comprising feeding the yeast protein extracts to a human or animal.

3. The use of claim 2 , wherein the yeast protein extract is used as a food supplement for weight control, for the elderly, or for sportspersons, comprising supplementing a diet with the yeast protein extract for weight control, for the elderly, or for sportspersons.

4. The method of claim 1 , wherein deaminase activity is also applied at step c).

5. The method of claim 1 , wherein the yeasts are selected from the group consisting of the species Saccharomyces, Pichia, Candida, Kluyveromyces, Yarrowia and Wickerhamomyces.

6. The method of claim 1 , wherein the yeasts are selected from the group consisting of Saccharomyces cerevisiae, Pichia jadinii and Kluyveromyces marxianus.

7. The method of claim 1 , wherein the enzymes used at step c) are used simultaneously.

8. The method of claim 1 , wherein the insoluble fraction collected at step d) also has a lipid content higher than 7%.

9. The method of claim 1 , wherein the insoluble fraction collected at step d) is treated with ethanol, a solvent or supercritical CO 2 to remove the lipids and increase the true protein content to 80%.

10. The method of claim 1 , wherein the yeast cream used at step a) contains a selenium-enriched yeast.

Assignees

• Lesaffre & Cie

Inventors

• Menin Rudy
• Spolaore Pauline
• Mouly Isabelle

Classifications

• A23J1/18 Primary

  from yeasts · CPC title

• A23L31/10

  Yeasts or derivatives thereof · CPC title

• A23L33/14

  Yeasts or derivatives thereof · CPC title

• C12N1/063

  of yeast · CPC title

• C12N1/066

  by physical processes · CPC title