Insecticidal proteins from plants and methods for their use
US-2016347799-A1 · Dec 1, 2016 · US
US11937604B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11937604-B2 |
| Application number | US-202117474028-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 13, 2021 |
| Priority date | Sep 13, 2021 |
| Publication date | Mar 26, 2024 |
| Grant date | Mar 26, 2024 |
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A nucleotide sequence is shown in SEQ ID NO.1. The gene encodes mannosyltransferase I. The gene plays an important role in the normal reproductive development of the Nilaparvata lugens (Stål). Inhibition of the function of the gene may reduce the survival rate of the Nilaparvata lugens (Stål) and hinder embryonic development. With respect to reduction of the survival rate of the Nilaparvata lugens (Stål) and hindering of embryonic development, the present invention can reduce the harm of the Nilaparvata lugens (Stål) to rice by killing the Nilaparvata lugens (Stål). By using the characteristic that the nucleotide sequence of a highly conserved target gene has no homology with the nucleotide sequence of natural enemies of the Nilaparvata lugens (Stål), RNA interference is performed at a nucleic acid level, to avoid the harm to non-target organisms such as natural enemies, thereby realizing green control of the Nilaparvata lugens (Stål) while controlling pests.
Opening claim text (preview).
The invention claimed is: 1. A method for reducing a survival rate of Nilaparvata lugens (Stål), comprising: injecting 0.05 μl of PIGM double-stranded RNA (dsPIGM) into the Nilaparvata lugens (Stål) for interference; the PIGM gene having a nucleotide sequence shown in SEQ ID NO.1; and inhibiting the gene encoding mannosyltransferase I.
Animals; Substances produced thereby or obtained therefrom · CPC title
Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins · CPC title
DNA or RNA fragments; Modified forms thereof (DNA or RNA not used in recombinant technology, C07H21/00); {Non-coding nucleic acids having a biological activity} · CPC title
Insecticides · CPC title
Insects · CPC title
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