Enzyme and method for assaying pentosidine using same

Patent metadata

Field	Value
Publication number	US-11932880-B2
Application number	US-201916975681-A
Country	US
Kind code	B2
Filing date	Feb 27, 2019
Priority date	Feb 27, 2018
Publication date	Mar 19, 2024
Grant date	Mar 19, 2024

Abstract

The present invention provides a protein having pentosidine oxidase activity, a method for measuring pentosidine comprising: contacting the protein with a specimen; and detecting a change caused by the contact, and the like.

First claim

The invention claimed is:

1. A method for measuring pentosidine, comprising:
contacting a protein with a specimen, wherein the protein is selected from the group consisting of:
(a) a protein consisting of an amino acid sequence as set forth in SEQ ID NO: 2 or 4;
(b) a protein encoded by a gene consisting of a nucleotide sequence as set forth in SEQ ID NO: 1, 3, 5 or 6;
(c) a protein comprising at least 90% sequence identity to SEQ ID NO: 2 or 4; and
(d) a protein encoded by a gene comprising at least 90% sequence identity to SEQ ID NO: 1, 3, 5 or 6; and

detecting a change caused by the contact.

2. The method according to claim 1 , wherein a change in an amount of oxygen, hydrogen peroxide or ammonia is detected in the detecting step.

3. The method according to claim 1 , wherein the protein is extracted from a filamentous fungus.

4. The method according to claim 1 , wherein the protein has an optimum pH of about 6.5 to 8.0.

5. The method according to claim 1 , wherein the protein has an optimum temperature of 37 to 50° C.

6. The method according to claim 1 , wherein 90% or more of the activity of the protein is retained after storage at 30° C. for 10 minutes.

7. A recombinant vector comprising the gene selected from the group consisting of:
(a) a gene encoding a protein consisting of an amino acid sequence as set forth in SEQ ID NO: 2 or 4;
(b) a gene consisting of a nucleotide sequence as set forth in SEQ ID NO: 1, 3, 5 or 6;
(c) a gene encoding a protein comprising at least 90% sequence identity to SEQ ID NO: 2 or 4; and
(d) a gene comprising at least 90% sequence identity to SEQ ID NO: 1, 3, 5 or 6.

8. A transformant comprising the vector according to claim 7 .

Assignees

• Kikkoman Corp

Inventors

• Marushima Kazuya
• Saito Yuka
• Tsukada Yuki
• Sato Takuya
• Araki Yasuko
• Ichiyanagi Atsushi

Classifications

• C12N9/0006 Primary

  acting on CH-OH groups as donors (1.1) · CPC title

• C12Q1/26

  involving oxidoreductase · CPC title

• C12Y101/03

  with a oxygen as acceptor (1.1.3) · CPC title

• C12N15/80 Primary

  for fungi · CPC title

• C12P3/00

  Preparation of elements or inorganic compounds except carbon dioxide {(recovery of carbon dioxides as by-products C12F3/02)} · CPC title