Two-photon excited fluorescence microscope for diagnosis of Alzheimer's disease (AD) and mild cognitive impairment (MCI), and pulse compressor including therein

Patent metadata

Field	Value
Publication number	US-11921273-B2
Application number	US-202117460478-A
Country	US
Kind code	B2
Filing date	Aug 30, 2021
Priority date	Oct 30, 2020
Publication date	Mar 5, 2024
Grant date	Mar 5, 2024

Abstract

Provided are a pulse compressor and a two-photon excited fluorescence microscope. The microscope includes a light source which generates a laser beam having a pulse, a pulse compressor which compresses the pulse of the laser beam, an objective lens which provides the laser beam to a specimen, and image sensors which receive the laser beam and obtain images of the specimen. The pulse compressor may include a grating plate, a corner cube provided on one side of the grating plate, and a retroreflector provided on the other side of the grating plate.

First claim

What is claimed is:

1. A two-photon excited fluorescence microscope comprising:
a light source configured to generate a laser beam;
a pulse compressor configured to compress a pulse of the laser beam;
an objective lens configured to direct the laser beam to a specimen;
image sensors configured to receive the laser beam and obtain surface and internal images of the specimen,
wherein the pulse compressor comprises:
a grating plate configured to disperse the laser beam;
a corner cube provided on a first side of the grating plate and configured to reflect the laser beam to the grating plate; and
a retroreflector provided on a second side of the grating plate and configured to re-reflect the laser beam, which has been transmitted through the grating plate, to the corner cube and the grating plate.

2. A two-photon excited fluorescence microscope comprising:
a light source configured to generate a laser beam;
a pulse compressor configured to compress a pulse of the laser beam;
an objective lens configured to provide the laser beam to a specimen; and
image sensors configured to receive the laser beam and obtain images of the specimen,
wherein the pulse compressor comprises:
a grating plate configured to disperse the laser beam;
a corner cube provided on a first side of the grating plate and configured to reflect the laser beam to the grating plate; and
a retroreflector provided on a second side of the grating plate and configured to re-reflect the laser beam, which has been transmitted through the grating plate, to the corner cube and the grating plate, and
wherein the grating plate comprises:
a first diffraction region;
a second diffraction region spaced apart from the first diffraction region;
a third diffraction region between the second diffraction region and the first diffraction region; and
a fourth diffraction region between the third diffraction region and the first diffraction region.

3. The two-photon excited fluorescence microscope of claim 2 ,
wherein the retroreflector is in contact with the second diffraction region and the third diffraction region, and
wherein the retroreflector is spaced apart from the first diffraction region and the fourth diffraction region.

4. The two-photon excited fluorescence microscope of claim 2 ,
wherein the pulse compressor further comprises a rotator, and
wherein the rotator is connected to a side wall of the grating plate adjacent to the first diffraction region to rotate the grating plate.

5. The two-photon excited fluorescence microscope of claim 2 ,
wherein the pulse compressor further comprises a cylinder, and
wherein the cylinder is provided between the third diffraction region and the fourth diffraction region to adjust a distance between the grating plate and the corner cube.

6. The two-photon excited fluorescence microscope of claim 1 , wherein the light source comprises a femtosecond pulse laser.

7. The two-photon excited fluorescence microscope of claim 1 , further comprising beam splitters provided between the pulse compressor and the objective lens.

8. The two-photon excited fluorescence microscope of claim 7 , wherein the beam splitters comprise:
a first beam splitter; and
a second beam splitter between the first beam splitter and the objective lens.

9. The two-photon excited fluorescence microscope of claim 8 , further comprising tube lenses between the pulse compressor and the first beam splitter.

10. The two-photon excited fluorescence microscope of claim 9 , further comprising planar mirrors between the tube lenses and the pulse compressor.

11. A pulse compressor comprising:
a grating plate configured to disperse a laser beam;
a corner cube provided on a first side of the grating plate and configured to reflect the laser beam to the grating plate;
a retroreflector provided on a second side of the grating plate and configured to re-reflect the laser beam, which has been transmitted through the grating plate, to the corner cube and the grating plate; and
a rotator connected to a side wall of the grating plate, the sidewall positioned between the first side of the grating plate and the second side of the grating plate,
wherein the rotator is configured to rotate the grating plate to adjust a dispersion value of the laser beam.

12. The pulse compressor of claim 11 , wherein the grating plate comprises:
a first diffraction region adjacent to the rotator;
a second diffraction region spaced apart from the first diffraction region;
a third diffraction region between the second diffraction region and the first diffraction region; and
a fourth diffraction region between the third diffraction region and the first diffraction region.

13. The pulse compressor of claim 12 ,
wherein the retroreflector is in contact with the second diffraction region and the third diffraction region, and
wherein the retroreflector is spaced apart from the first diffraction region and the fourth diffraction region.

14. The pulse compressor of claim 12 , further comprising a cylinder provided between the third diffraction region and the fourth diffraction region,
wherein the cylinder is configured to adjust a distance between the grating plate and the corner cube.

15. The pulse compressor of claim 12 , further comprising a spatial light modulator provided between the grating plate and the corner cube.

16. The pulse compressor of claim 15 , wherein the spatial light modulator is provided adjacent to the fourth diffraction region.

Assignees

• Electronics & Telecommunications Res Inst

Inventors

• Lee Sang Kyun
• Song Dong Hoon
• Seo Hong-Seok
• Huh Chul

Classifications

• G02B21/0076 Primary

  arrangements using fluorescence or luminescence · CPC title

• A61B5/4088 Primary

  Diagnosing of monitoring cognitive diseases, e.g. Alzheimer, prion diseases or dementia · CPC title

• G02B21/361

  Optical details, e.g. image relay to the camera or image sensor (G02B21/364 takes precedence; illumination details G02B21/06 and subgroups) · CPC title

• H01S3/0057

  Temporal shaping, e.g. pulse compression, frequency chirping (soliton generation and propagation G02F1/3513, H01S3/063 and H01S3/108) · CPC title

• G01N21/6458

  Fluorescence microscopy (fluorescence microscopes per se G02B21/0076 and G02B21/16) · CPC title