Insecticidal polypeptides and uses thereof

Patent metadata

Field	Value
Publication number	US-11920146-B2
Application number	US-202217931210-A
Country	US
Kind code	B2
Filing date	Sep 12, 2022
Priority date	Dec 19, 2017
Publication date	Mar 5, 2024
Grant date	Mar 5, 2024

Abstract

The disclosure provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidopteran and Coleopteran. Particular embodiments of the disclosure provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

First claim

That which is claimed:

1. A recombinant polynucleotide encoding an insecticidal polypeptide comprising an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 268, wherein the polynucleotide is operably linked to a heterologous regulatory element.

2. The recombinant polynucleotide of claim 1 , wherein the polynucleotide is SEQ ID NO: 267.

3. The recombinant polynucleotide of claim 1 , wherein the polynucleotide is codon optimized for expression in an agricultural crop.

4. A DNA construct comprising the recombinant polynucleotide of claim 1 .

5. A host cell comprising the recombinant polynucleotide of claim 1 .

6. A host cell comprising the DNA construct of claim 4 .

7. A transgenic plant comprising the polynucleotide of claim 1 .

8. A transgenic plant comprising the DNA construct of claim 4 .

9. A method of inhibiting growth or killing an insect pest or pest population, comprising contacting the insect pest with the insecticidal polypeptide of claim 1 .

10. A method of inhibiting growth or killing an insect pest or pest population comprising expressing in a plant the polynucleotide of claim 1 .

11. A method for controlling a pest infestation comprising providing in the diet of the pest the transgenic plant of claim 7 or a part thereof.

12. A method for improving the yield of a crop comprising growing the transgenic plant of claim 7 , wherein the yield of the crop is increased in the presence of an insect pest relative to the crop not comprising said transgenic plant.

13. The method of claim 9 , wherein the insect pest or pest population is resistant to at least one Cry insecticidal protein.

14. The host cell of claim 5 , wherein the host cell is a bacterial cell or a plant cell.

15. The host cell of claim 14 , wherein the plant cell is a monocot or a dicot plant cell.

16. The transgenic plant of claim 7 , wherein the plant is selected from maize, soybean, wheat, rice, sorghum, sunflower, barley, sugarcane, potatoes, tomatoes, cotton, Brassica spp., peanut, or alfalfa.

17. The transgenic plant of claim 7 , further comprising an additional polynucleotide of interest selected from a polynucleotide encoding a polypeptide having pesticidal activity, insecticidal activity, herbicide resistance, or disease resistance.

Assignees

• Pioneer Hi Bred Int

Inventors

• Abad Andre R
• Dong Hua
• Lo Sue B
• Poland Brad
• Shi Xiaomei
• Wang Jimei
• Wolfe Thomas Chad
• Zhou Lan

Classifications

• C12N15/8286 Primary

  for insect resistance · CPC title

• C07K14/325 Primary

  Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title