Method for fabricating highly elastic porous polymer membrane, method for manufacturing cell culture system using porous polymer membrane fabricated by the fabrication method and cell culture system manufactured by the manufacturing method

Patent metadata

Field	Value
Publication number	US-11920118-B2
Application number	US-202017081723-A
Country	US
Kind code	B2
Filing date	Oct 27, 2020
Priority date	Mar 30, 2020
Publication date	Mar 5, 2024
Grant date	Mar 5, 2024

Abstract

Provided are a method for fabricating a highly elastic porous polymer membrane, a method for manufacturing a cell culture system using a porous polymer membrane fabricated by the fabrication method, and a cell culture system manufactured by the manufacturing method. The porous polymer membrane can be fabricated by spin coating a mixture solution containing a biodegradable elastomeric polymer and a water-soluble moisture absorbent in an optimum ratio while maintaining a high relative humidity. The porous polymer membrane has a uniform thickness and a uniform pore size and is highly elastic. In addition, the thickness and pore size of the porous polymer membrane can be controlled. The porous polymer membrane can induce active cell-cell interaction during cell co-culture due to its high porosity. The porous polymer membrane enables control over cell alignment or array due to its high biocompatibility and elasticity. The porous polymer membrane can be utilized in a platform for inducing stem cell differentiation, a lab-on-a-chip, a synthetic skin simulant platform, etc.

First claim

What is claimed is:

1. A method for fabricating a porous polymer membrane, comprising:
mixing a biodegradable elastomeric polymer with a water-soluble moisture absorbent in an organic solvent to prepare a mixture solution; and
applying the mixture solution to a substrate in a closed chamber, followed by spin coating at a relative humidity of 65 to 90%,
wherein the biodegradable elastomeric polymer is poly(L-lactide-co-caprolactone),
wherein a molar ratio of the L-lactide to the caprolactone in the poly(L-lactide-co-caprolactone) is 1-9:9-1,
wherein the poly(L-lactide-co-caprolactone) has
a number average molecular weight (Mn) of 50,000 to 500,000 g/mol, and
a weight average molecular weight (Mw) of 50,000 to 800,000 g/mol,

wherein a strain of the porous polymer membrane is 30 to 60% at a stretching temperature of 20 to 40° C.

2. The method according to claim 1 , wherein the water-soluble moisture absorbent is selected from the group consisting of LiCl, CaCl 2 , ZnCl 2 , KOH, NaOH, MgCl 2 , FeCl 3 , K 2 CO 3 , and mixtures thereof.

3. The method according to claim 1 , wherein the organic solvent is selected from the group consisting of tetrahydrofuran, dimethylformamide, diethylformamide, N-methyl-2-pyrrolidone, dimethyl sulfoxide, dimethylacetamide, methanol, ethanol, chloroform, dichloromethane, and mixtures thereof.

4. The method according to claim 1 , wherein the biodegradable elastomeric polymer is mixed with the water-soluble moisture absorbent in a weight ratio of 1:0.05-0.4 in the organic solvent to prepare the mixture solution.

5. The method according to claim 1 , wherein the relative humidity is adjusted to 70 to 85% with a supersaturated salt solution.

6. The method according to claim 1 , wherein the spin coating is performed at a speed of 1000 to 4000 rpm for 10 seconds to 1 minute.

7. The method according to claim 1 , wherein the porous polymer membrane has a thickness of 300 nm to 3 μm, a pore size of 100 nm to 5 μm, and a porosity of 20 to 80%.

8. The method according to claim 1 , wherein the biodegradable elastomeric polymer is poly(L-lactide-co-caprolactone), poly(L-lactide-co-glycolide) or a mixture thereof, the water-soluble moisture absorbent is LiCl, the organic solvent is tetrahydrofuran, the biodegradable elastomeric polymer is mixed with the water-soluble moisture absorbent in a weight ratio of 1:0.1-0.3 in the organic solvent to prepare the mixture solution, the relative humidity is adjusted to 74 to 76% with a supersaturated salt solution, the spin coating is performed at a speed of 2800 to 3400 rpm for 20 seconds to 40 seconds, and the porous polymer membrane has a thickness of 700 nm to 960 nm, a pore size of 900 nm to 1.2 μm, and a porosity of 35 to 45%.

9. The method according to claim 8 , wherein the biodegradable elastomeric polymer is poly(L-lactide-co-caprolactone), the water-soluble moisture absorbent is LiCl, the organic solvent is tetrahydrofuran, the biodegradable elastomeric polymer is mixed with the water-soluble moisture absorbent in a weight ratio of 1:0.2 in the organic solvent to prepare the mixture solution, the relative humidity is adjusted to 75% with a supersaturated salt solution, the spin coating is performed at a speed of 3000 rpm for 30 seconds, and the porous polymer membrane has a thickness of 960 nm, a pore size of 1 μm, and a porosity of 37%.

Assignees

• Korea Inst Sci & Tech

Inventors

• Jung Young Mee
• Kim Soo Hyun
• Chung Justin Jihong
• Yoo Jin
• Kim Tae Hee

Classifications

• C12M29/04 Primary

  Filters; Permeable or porous membranes or plates, e.g. dialysis · CPC title

• C12M23/26

  flexible (flexible containers for laboratory use B01L3/505) · CPC title

• C12M35/08 Primary

  Chemical, biochemical or biological means, e.g. plasma jet, co-culture · CPC title

• C08J9/0066 Primary

  Use of inorganic compounding ingredients · CPC title

• C12M25/02

  Membranes; Filters (filters or filtration in general B01D24/00-B01D41/00) · CPC title