Compact detection module for flow cytometers

Patent metadata

Field	Value
Publication number	US-11913868-B2
Application number	US-202117302651-A
Country	US
Kind code	B2
Filing date	May 10, 2021
Priority date	Jul 25, 2016
Publication date	Feb 27, 2024
Grant date	Feb 27, 2024

Abstract

In one embodiment, a flow cytometer is disclosed having a compact light detection module. The compact light detection module includes an image array with a transparent block, a plurality of micro-mirrors in a row coupled to a first side of the transparent block, and a plurality of filters in a row coupled to a second side of the transparent block opposite the first side. Each of the plurality of filters reflects light to one of the plurality of micro-mirrors and passes light of a differing wavelength range and each of the plurality of micro-mirrors reflects light to one of the plurality of filters, such that incident light into the image array zigzags back and forth between consecutive filters of the plurality of filters and consecutive micro-mirrors of the plurality of micro-mirrors. A radius of curvature of each of the plurality of micro-mirrors images the fiber aperture onto the odd filters and collimates the light beam on the even filters.

First claim

What is claimed is:

1. An apparatus comprising:
a first light detection module to demultiplex a first fluorescent light in a first range of wavelengths, the first light detection module including
a first plurality of detectors in a first row;
a first transparent block adjacent the first plurality of detectors,
a first plurality of micro-mirrors coupled to a first side of the first transparent block, and
a first plurality of filters in a first row coupled to a second side of the first transparent block opposite the first side, each of the first plurality of filters to reflect light to one of the first plurality of micro-mirrors and pass light of a different wavelength range towards one of the first plurality of detectors.

2. The apparatus of claim 1 , further comprising:
a second light detection module to demultiplex a second fluorescent light in a second range of wavelengths, the second light detection module including
a second plurality of detectors in a second row;
a second transparent block adjacent the second plurality of detectors,
a second plurality of micro-mirrors coupled to a first side of the second transparent block, and
a second plurality of filters in a second row coupled to a second side of the second transparent block opposite the first side, each of the second plurality of filters to reflect light to one of the second plurality of micro-mirrors and pass light of a different wavelength range towards one of the second plurality of detectors.

3. The apparatus of claim 2 , further comprising:
an input channel coupled in light communication with the first light detection module and the second light detection module, the input channel to receive a fluorescent light beam and split it into the first fluorescent light in the first range of wavelengths and the second fluorescent light in the second range of wavelengths.

4. The apparatus of claim 3 , wherein
the input channel includes
a wavelength beam splitter (other than power beam splitter) to split the fluorescent light beam into the first fluorescent light and the second fluorescent light.

5. The apparatus of claim 3 , wherein
the input channel further includes
a collimating lens to receive the fluorescent light from an optical fiber and collimate the fluorescent light, and
a blocking filter to reject a laser light used to stimulate fluorochromes in the fluorescent light and pass the fluorescent light to the beam splitter.

6. The apparatus of claim 5 , wherein
the input channel further includes
a first focusing lens in light communication with the beam splitter, the first focusing lens to focus the first fluorescent light onto a first filter of the first plurality of filters, and
a second focusing lens in light communication with the beam splitter, the second focusing lens to focus the collimated light with the second range of wavelengths onto a first filter of the second plurality of filters.

Assignees

• Cytek Biosciences Inc

Inventors

• Yan Ming
• Hsieh Yung-Chieh
• Vrane David
• Chase Eric

Classifications

• G01N15/1436 Primary

  the optical arrangement forming an integrated apparatus with the sample container, e.g. a flow cell · CPC title

• G01J1/08

  Arrangements of light sources specially adapted for photometry {standard sources, also using luminescent or radioactive material} · CPC title

• G01J3/021

  using plane or convex mirrors, parallel phase plates, or particular reflectors · CPC title

• G01J3/0208

  using focussing or collimating elements, e.g. lenses or mirrors; performing aberration correction · CPC title

• G01J3/0218

  using optical fibers · CPC title