Automatic analyzer and analysis method

Patent metadata

Field	Value
Publication number	US-11906406-B2
Application number	US-201816618630-A
Country	US
Kind code	B2
Filing date	Apr 5, 2018
Priority date	Jun 2, 2017
Publication date	Feb 20, 2024
Grant date	Feb 20, 2024

Abstract

Provided is an automatic analysis technique that prevents liquid contained in a reaction container from locally contacting with liquid added afterward and has less occurrence frequency of equipment malfunction and high performance. An automatic analyzer ( 1 ) includes: a reaction container disk ( 120 ) that holds a reaction container ( 116 ); a stirring mechanism ( 124 ) that stirs liquid contained in the reaction container ( 116 ) in a non-contact manner; a carrying mechanism ( 125 ) that carries the reaction container ( 116 ) between the reaction container disk ( 120 ) and the stirring mechanism ( 124 ); a reagent disk ( 122 ) that holds a reagent container ( 121 ); and a reagent dispensing mechanism ( 123 ) that suctions and discharges a reagent contained in the reagent container ( 121 ), the stirring mechanism ( 124 ) is provided at a position where the reagent dispensing mechanism ( 123 ) discharges the reagent, and the reagent dispensing mechanism ( 123 ) discharges the reagent, which is sucked from the reagent container ( 121 ), to the reaction container ( 116 ) installed in the stirring mechanism ( 124 ).

First claim

The invention claimed is:

1. An analysis method comprising:
a step in which a carrying mechanism carries a reaction container containing a sample from a reaction container disk to a stirring mechanism and installs the reaction container in the stirring mechanism;
a step in which the stirring mechanism, including an ultrasonic wave emission apparatus configured to emit ultrasonic waves from a piezoelectric element to a side surface of the reaction container to form a rotational flow of a liquid in the reaction container, starts to stir the sample contained in the reaction container without contacting the sample;
a step in which a reagent dispensing mechanism intermittently discharges a reagent to the reaction container a plurality of times after the stirring mechanism starts the stirring, and the stirring mechanism stirs the liquid contained in the reaction container while the reagent dispensing mechanism stops discharging the reagent to the reaction container;
a step in which the carrying mechanism returns the reaction container, to which the reagent is discharged, from the stirring mechanism to the reaction container disk;
a step in which a detector measures a light emission amount of the reagent; and
a step in which a controller controls analysis of the light emission amount of the reagent;
wherein a stirring speed of the stirring mechanism is controlled by controlling an intensity of ultrasonic waves emitted to the reaction container, and the stirring mechanism stirs the liquid contained in the reaction container such that a relation of U N ≤U N+1 is established, where U N represents a first stirring speed performed after the reagent dispensing mechanism discharges the reagent N times and U N+1 represents a second stirring speed performed after the reagent dispensing mechanism discharges the reagent (N+1) times.

2. The analysis method according to claims 1 , wherein
the stirring mechanism stirs the liquid contained in the reaction container before the reagent dispensing mechanism completely discharges a predetermined amount of the reagent to the reaction container.

3. The analysis method according to claims 1 , wherein
the reagent dispensing mechanism changes an amount per unit time of the reagent to be discharged, based on a total amount of the reagent discharged to the reaction container or a period of time elapsed from the start of discharge of the reagent to the reaction container.

4. The analysis method according to claims 1 , wherein
the reagent dispensing mechanism discharges the reagent such that a relation of V A ≤V B is established, where V A represents an amount per unit time of the liquid to be discharged after T A seconds from the start of discharge of the reagent to the reaction container, and V B represents an amount per unit time of the liquid to be discharged after T B seconds (where T A ≤T B ) from the start of discharge of the reagent to the reaction container.

5. The analysis method according to claim 1 , wherein
the reagent dispensing mechanism discharges the reagent to the reaction container several times such that a relation of V N ≤V N+1 is established, where V N represents an amount of the liquid to be discharged at an N-th time and V N+1 represents an amount of the liquid to be discharged at an (N+1)-th time.

6. The analysis method according to claim 1 , wherein
a stirring speed of the stirring mechanism is controlled by controlling an intensity of ultrasonic waves emitted to the reaction container, and changes to the stirring speed are determined based on a total amount of the reagent discharged to the reaction container by the reagent dispensing mechanism or a period of time elapsed from the start of discharge of the reagent to the reaction container.

7. The analysis method according to claim 1 , wherein
the reaction container is installed in the stirring mechanism and the stirring mechanism is temperature-controlled such that the reaction container maintains a temperature within a predetermined range.

Assignees

• Hitachi High Tech Corp
• Roche Diagnostics Operations Inc

Inventors

• Ebihara Daisuke
• Matsuoka Shinya
• Sakazume Taku
• Shimazaki Yuzuru

Classifications

• G01N1/38 Primary

  Diluting, dispersing or mixing samples · CPC title

• G01N35/025

  having a carousel or turntable for reaction cells or cuvettes · CPC title

• G01N35/04

  Details of the conveyor system {(G01N35/021 - G01N35/028 take precedence)} · CPC title

• G01N2035/00514

  Stationary mixing elements · CPC title

• G01N2035/00524

  Mixing by agitating sample carrier · CPC title