Amylases, nucleic acids encoding them and methods for making and using them
US-9249400-B2 · Feb 2, 2016 · US
US11903397B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11903397-B2 |
| Application number | US-201515528998-A |
| Country | US |
| Kind code | B2 |
| Filing date | Nov 24, 2015 |
| Priority date | Nov 24, 2014 |
| Publication date | Feb 20, 2024 |
| Grant date | Feb 20, 2024 |
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There is provided a method of treating hay for preventing and/or reducing heat damage in high moisture hay and as well to preserve the same, the method including adding to hay a hay preservative including a hay preserving and heat reducing effective amount of at least one enzyme having a chitinase activity, either alone or in combination with a hay preserving and heat reducing effective amount of a yeast of the genus Pichia or a bacteria of the genus Pediococcus . There is also provided a method of treating hay for preventing and/or reducing heat damage in high moisture hay and as well to preserve the same, the method including adding to hay a hay preservative including a hay preserving and heat reducing effective amount of yeast of the genus Pichia.
Opening claim text (preview).
The invention claimed is: 1. A hay preservative composition comprising an enzyme with chitinase activity and a yeast of the genus Pichia or a bacteria of the genus Pediococcus , wherein the chitinase activity is in the range of about 6 U to 250 U per ton of hay to be treated and the amount of either Pichia or Pediococcus is 10 5 to 10 15 viable organisms per ton of hay to be treated and wherein the composition is effective to reduce the heat of the hay. 2. The hay preservative composition of claim 1 , wherein the Pichia is Pichia anomala. 3. The hay preservative composition of claim 2 , wherein the Pichia anomala is Pichia anomala (Accession number DBVPG 3003). 4. The hay preservative composition of claim 1 , wherein the Pediococcus is Pediococcus pentosaceus. 5. The hay preservative composition of claim 4 , wherein the Pediococcus pentosaceus is Pediococcus pentosaceus BTC328 (Accession number NCIMB 12674) or Pediococcus pentosaceus BTC401 (Accession number NCIMB 12675). 6. The hay preservative composition of claim 1 , further comprising at least one enzyme having a Pectin lyase activity, a Glucanase activity, or a mixture thereof. 7. The hay preservative composition of claim 1 comprising a high moisture hay. 8. The hay preservative composition of claim 7 further comprising an effective amount of Pichia. 9. The hay preservative composition of claim 8 , wherein the Pichia is Pichia anomala. 10. The hay preservative composition of claim 9 , wherein the Pichia anomala is Pichia anomala (Accession number DBVPG 3003). 11. A method of reducing heat damage in high moisture hay comprising adding the composition of claim 1 to high moisture hay. 12. The method of to claim 11 , wherein the Pichia is Pichia anomala. 13. The method of claim 12 , wherein the Pichia anomala is Pichia anomala (Accession number DBVPG 3003). 14. The method of claim 11 , wherein the Pediococcus is Pediococcus pentosaceus. 15. The method of claim 14 , wherein the Pediococcus pentosaceus is Pediococcus pentosaceus BTC328 (Accession number NCIMB 12674) or Pediococcus pentosaceus BTC401 (Accession number NCIMB 12675). 16. The method of claim 11 , wherein the heat preserving composition further comprises at least one enzyme having a Pectin lyase activity, a Glucanase activity, or a mixture thereof.
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Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves {or protection against physical influences such as UV radiation using chemical compositions} (preservation or chemical ripening of fruit or vegetables A23B7/00); Grafting wax · CPC title
Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs · CPC title
Bacteria; Culture media therefor · CPC title
Bacterial isolates · CPC title
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