Silver nanocluster fluorescent nanotube, a preparation method and its application in the detection of arginine

Patent metadata

Field	Value
Publication number	US-11891553-B2
Application number	US-202218049723-A
Country	US
Kind code	B2
Filing date	Oct 26, 2022
Priority date	Nov 25, 2021
Publication date	Feb 6, 2024
Grant date	Feb 6, 2024

Abstract

A preparation process of atomically precise nine-nuclear silver nanoclusters (Ag9-NCs) fluorescent nanotube and its application in the detection of arginine (Arg), the fluorescent nanotube is formed by supramolecular self-assembly of Ag9-NCs and peptide (DD-5); the fluorescent nanotube prepared by the present invention has good luminescence performance due to its highly ordered structure, the quantum yield is 8.11%, and the fluorescence lifetime is 6.10 μs; after adding Arg, the highly ordered structure is destroyed, resulting in fluorescent quenching; the preparation method of the Ag9-NCs fluorescent nanotube of this invention is simple, the cost is low; at the same time, the detection method is fast and easy to observe.

First claim

What is claimed is:

1. A silver nanocluster (Ag 9 -NCs) fluorescent nanotube, characterized in that, the Ag 9 -NCs fluorescent nanotube is obtained by a supramolecular self-assembly process of Ag 9 under an induction of DD-5;
wherein the DD-5 is formed by a polymerization of five aspartic acids; the Ag 9 is nine-nuclear Ag with Ag as core and 2-mercaptobenzoic acid (H 2 mba) as ligand; and
the supramolecular self-assembly process is to mix Ag 9 aqueous solution with DD-5, vortex, and let it stand for 8 hours in 20° C. incubator to obtain the Ag 9 -NCs fluorescent nanotube;
wherein the Ag 9 aqueous solution is produced by dispersing AgNO 3 and H 2 mba in water with ultrasonic treatment, and adding NH 3 ·H 2 O; and the Ag 9 -NCs fluorescent nanotube is sensitivity to L-Arg only, which is specifically used for detecting L-Arg.

2. The silver nanocluster (Ag 9 -NCs) fluorescent nanotube according to claim 1 , characterized in that, the Ag 9 -NCs fluorescent nanotube has a diameter of 30-50 nm and a length of 5-20 μm.

3. The silver nanocluster (Ag 9 -NCs) fluorescent nanotube according to claim 1 , characterized in that, fluorescence spectrum of the Ag 9 -NCs fluorescent nanotube shows that excitation wavelength is 400-550 nm, and emission wavelength is 550-800 nm.

4. The silver nanocluster (Ag 9 -NCs) fluorescent nanotube according to claim 1 , characterized in that, fluorescence lifetime of the Ag 9 -NCs fluorescent nanotube is 6.10 μs, and quantum yield is 8.11%.

5. The silver nanocluster (Ag 9 -NCs) fluorescent nanotube according to claim 1 , wherein concentration of AgNO 3 is 1 mmol·L −1 , and concentration of H 2 mba is 1 mmol·L −1 ; molar ratio of AgNO 3 and H 2 mba is 1:1.

6. The silver nanocluster (Ag 9 -NCs) fluorescent nanotube according to claim 1 , wherein ultrasonic frequency of the ultrasonic treatment is 30-50 kHz, ultrasonic power is 80 W, and time is 20-30 minutes.

7. The silver nanocluster (Ag 9 -NCs) fluorescent nanotube according to claim 1 , wherein mass concentration of NH 3 ·H 2 O is 25%.

8. The silver nanocluster (Ag 9 -NCs) fluorescent nanotube according to claim 1 , wherein amount of NH 3 ·H 2 O added is until precipitate is completely dissolved.

9. The silver nanocluster (Ag 9 -NCs) fluorescent nanotube according to claim 1 , wherein final molar concentration of the DD-5 is 50-80 mmol·L −1 and final molar concentration of Ag 9 -NCs is 5 mmol·L −1 .

10. The silver nanocluster (Ag 9 -NCs) fluorescent nanotube according to claim 1 , wherein vortex time is 20-30 seconds during the supramolecular self-assembly process.

Assignees

• Univ Shandong

Inventors

• Xin Xia
• Wang Wenjuan
• Sun Di
• Wang Zhi

Classifications

• C09K11/58 Primary

  containing copper, silver or gold · CPC title

• B82Y5/00

  Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery · CPC title

• B82Y15/00

  Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors · CPC title

• C01B32/16

  Preparation · CPC title

• C09K11/06 Primary

  containing organic luminescent materials · CPC title