Automated pipetting equipment, multi-channel pipetting assembly, and pipetting structure

Patent metadata

Field	Value
Publication number	US-11890612-B2
Application number	US-202117302271-A
Country	US
Kind code	B2
Filing date	Apr 29, 2021
Priority date	Feb 5, 2021
Publication date	Feb 6, 2024
Grant date	Feb 6, 2024

Abstract

A multi-channel pipetting assembly includes a linkage member and a plurality of pipetting structures arranged in parallel. Each pipetting structure includes a pipette body, a piston rod, a piston tube, and an elastic element. The pipette body has a plurality of air chambers with different inner diameters, and the air chambers are arranged axially and communicate with each other. The piston rod is fixed to the linkage member. The piston tube is sleeved on the piston rod. The piston rod and the piston tube are located in the pipette body, and are axially and reciprocatingly movable in the pipette body. The piston rod matches the air chamber with the smallest inner diameter, and the piston tube matches the remaining air chambers. The elastic element is telescopically sleeved on the piston rod and corresponds to the piston tube. Therefore, a plurality of volume ranges meeting the accuracy requirements can be provided.

First claim

What is claimed is:

1. Automated pipetting equipment, adapted for pipetting between a plurality of workbenches, wherein the automated pipetting equipment comprises:
a movable mechanism adapted to move between any two of the plurality of workbenches;
a multi-channel pipetting assembly disposed on the movable mechanism and comprising:
a bracket disposed on the movable mechanism;
a linkage member; and
a plurality of pipetting structures disposed on the bracket and the linkage member in parallel, wherein each of the pipetting structures comprises:
a pipette body disposed on the bracket and including an air displacement tube and an air outlet end, wherein the air displacement tube is engaged with the air outlet end, the air displacement tube has a plurality of air chambers with different inner diameters, the plurality of air chambers are arranged axially according to the inner diameters and communicate with each other, the air outlet end is configured to be removably sleeved and coupled with a tip, the air outlet end has an air outlet passage, and the air outlet passage penetrates the air outlet end in the axial direction and communicates with the air chamber;
a piston rod fixed to the linkage member, being axially and reciprocatingly movable in the pipette body, the outer diameter of the piston rod being substantially equal to the inner diameter of the air chamber of the plurality of air chambers that has a smallest inner diameter;
at least one piston tube located in the pipette body, sleeved on the piston rod, the outer diameter of the at least one piston tube being substantially equal to the inner diameter of the remaining air chambers of the plurality of air chambers, and each being axially and reciprocatingly movable in the air displacement tube of the pipette body to enter or leave the matching air chamber; and
at least one elastic element corresponding to the at least one piston tube and telescopically sleeved on the piston rod; and
a driving module disposed on the movable mechanism and adapted to drive the linkage member to axially reciprocate.

2. The automated pipetting equipment according to claim 1 , wherein one end of each of the elastic elements is fixed to the piston rod, and the other end of each of the elastic elements is fixed to the corresponding piston tube.

3. The automated pipetting equipment according to claim 2 , wherein when a lower end of the piston rod moves in any of the remaining air chambers, the elastic element fixed to the piston tube corresponding to the air chamber maintains an original length.

4. The automated pipetting equipment according to claim 1 , wherein in each of the pipetting structures, a sealing member is provided between an outermost side of the at least one piston tube and an inner wall of the pipette body.

5. The automated pipetting equipment according to claim 4 , wherein a lower end of the pipette body is the air outlet end, and the sealing member is fixed to an upper end of the pipette body with a sealing cover.

6. The automated pipetting equipment according to claim 1 , wherein in each of the pipetting structures, a sealing member is provided between any two of the at least one piston tube and the piston rod that are adjacent to each other.

7. The automated pipetting equipment according to claim 6 , wherein each of the piston tubes comprises a piston body and a cover body, and the cover body fixes the sealing member to the piston body.

8. The automated pipetting equipment according to claim 1 , wherein the piston rod comprises:
a piston section matching the air chamber of the plurality of air chambers that has the smallest inner diameter;
a fixing section fixed to the linkage member; and
a connecting section coupled between the piston section and the fixing section.

9. The automated pipetting equipment according to claim 1 , wherein the driving module comprises:
a motor; and
a shaft connected between the motor and the linkage member.

10. A multi-channel pipetting assembly, comprising:
a linkage member; and
a plurality of pipetting structures disposed on the linkage member in parallel, wherein each of the pipetting structures comprises:
a pipette body including an air displacement tube and an air outlet end, wherein the air displacement tube is engaged with the air outlet end, the air displacement tube has a plurality of air chambers with different inner diameters, the plurality of air chambers are arranged axially according to the inner diameters and communicate with each other, the air outlet end is configured to be removably sleeved and coupled with a tip, the air outlet end has an air outlet passage, and the air outlet passage penetrates the air outlet end in the axial direction and communicates with the air chamber;
a piston rod fixed to the linkage member, located in the pipette body, being axially and reciprocatingly movable in the pipette body, the outer diameter of the piston rod being substantially equal to the inner diameter of the air chamber of the plurality of air chambers that has a smallest inner diameter;
at least one piston tube located in the pipette body, sleeved on the piston rod, the outer diameter of the at least one piston tube being substantially equal to the inner diameter of the remaining air chambers of the plurality of air chambers, and each being axially and reciprocatingly movable in the air displacement tube of the pipette body to enter or leave the matching air chamber; and
at least one elastic element corresponding to the at least one piston tube and telescopically sleeved on the piston rod.

11. The multi-channel pipetting assembly according to claim 10 , wherein one end of each of the elastic elements is fixed to the piston rod, and the other end of each of the elastic elements is fixed to the corresponding piston tube.

12. The multi-channel pipetting assembly according to claim 11 , wherein when a lower end of the piston rod moves in any of the remaining air chambers, the elastic element fixed to the piston tube corresponding to the air chamber maintains an original length.

13. The multi-channel pipetting assembly according to claim 10 , wherein in each of the pipetting structures, a sealing member is provided between an outermost side of the at least one piston tube and an inner wall of the pipette body.

14. The multi-channel pipetting assembly according to claim 13 , wherein a lower end of the pipette body is the air outlet end, and the sealing member is fixed to an upper end of the pipette body with a sealing cover.

15. The multi-channel pipetting assembly according to claim 10 , wherein in each of the pipetting structures, a sealing member is provided between any two of the at least one piston tube and the piston rod that are adjacent to each other.

16. The multi-channel pipetting assembly according to claim 15 , wherein each of the piston tubes comprises a piston body and a cover body, and the cover body fixes the sealing member to the piston body.

17. The multi-channel pipetting assembly according to claim 11 , wherein the piston rod comprises:
a piston section matching the air chamber of the plurality of air chambers that has the smallest inner diameter;
a fixing section fixed to the linkage member; and
a connecting section coupled between the piston section and the fixing section.

Assignees

• Wistron Corp

Inventors

• Weng Chi-Neng
• Lin Chih-Kuan
• Peng shao-wei
• Chu Chih-Ying

Classifications

• B01L3/0217 Primary

  of the plunger pump type · CPC title

• G01N35/1065 Primary

  Multiple transfer devices · CPC title

• B01L2200/025

  Align devices or objects to ensure defined positions relative to each other · CPC title

• B01L2200/14

  Process control and prevention of errors · CPC title

• B01L2400/0478

  pistons · CPC title